1. Straub TM, Honer zu Bentrup K, OroszCoghlan P, Dohnalkova A, Mayer BK, Batholomew RA, et al. In vitro cell culture assay for human noroviruses. Emerg Infect Dis. 2007;13:396–403. Available from http://www.cdc.gov/eid/content/13/3/396. htm 2. Rochelle PA, Marshall MM, Mead JR, Johnson AM, Korich DG, Rosen JS, et al. Comparison of in vitro cell culture and mouse assay for measuring infectivity...

Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted unde...

The collagenase digestion phase of islet isolation is variable and unpredictable. This results partly from the vagaries of collagenase itself but also from the complex effects of organ retrieval and storage on collagenase digestion. Improvements in the purification of islets by density gradient centrifugation will not result from the production of new density gradient media, but rather from the...

An in vitro root explant tissue culture technique is described for determining susceptibility of tomato (Lycopersicon esculentum Mill.) breeding lines and cultivars to the root-knot nematode Meloidogyne incognita. Root explants were taken from 2-day-old seedlings cultured for 30 days at 28 C on Gamborg's B-5 medium with or without nematode inoculum. The remaining portion of the root and stem fr...

The orientation imposed by the subject of this monograph leads us to emphasize those aspects of the chemistry of infection that may serve our understanding of the morphology of infection. Indeed, much of the effort expended in this field has been justified under a hypothesis that the genesis of the cytopathic effect is a biochemical lesion. How that lesion is inflicted by the virus, the ensuing...

Introduction A perfusion-based process was developed to increase the split ratio during the scale-up of CHO-STM cell cultures. Fedbatch cultures were inoculated with cells propagated in either batch or perfusion cultures. All cultures were grown in disposable CellbagTM bioreactors using the WAVE Bioreactor system. Cell concentrations of 4.8 × 10 cells/mL were achieved in the perfusion culture, ...

The importance of developing artificial media that can be used in the place of serum for maintaining the life of tissues and organs outside the body hardly needs to be emphasized. Many of the studies for which the organ culture technique 1 was developed, as well as others that can be carried on by the simpler methods of tissue culture, depend for their success on the creation of suitable artifi...

Introduction A perfusion-based process was developed to increase the split ratio during the scale-up of CHO-STM cell cultures. Fedbatch cultures were inoculated with cells propagated in either batch or perfusion cultures. All cultures were grown in disposable CellbagTM bioreactors using the WAVE Bioreactor system. Cell concentrations of 4.8 × 10 cells/mL were achieved in the perfusion culture, ...

BACKGROUND Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. METHODS Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static...