In the process of oocyte maturation, gonadotrophins are believed as main stimulators for oocyte meiosis resumption. However, which gonadotrophin (i.e. FSH or LH) is the key hormone in this process is still unknown. This study indicated a close relationship between LH and FSH on activating meiotic maturation of oocyte in vitro. FSH efficiently induced oocyte meiosis at concentration of 50 IU/L, ...

BACKGROUND Human sperm chemotaxis to pre-ovulatory follicular fluid is well established in vitro. However, it is not known whether the female's oocyte-cumulus complex secretes sperm chemoattractants subsequent to ovulation (for enabling sperm chemotaxis within the Fallopian tube) and, if so, which of these cell types--the oocyte or the cumulus oophorus--is the physiological origin of the secret...

BACKGROUND In the cow, lysophosphatidic acid (LPA) acts as an auto-/paracrine factor, through its receptors LPAR1-4, on oocytes and cumulus cells during in vitro maturation (IVM). The aim of the present work was to determine the effect of LPA during IVM of bovine oocytes on: 1) oocyte maturation; 2) apoptosis of COCs; 3) expression of genes involved in developmental competence and apoptosis in ...

Bidirectional communication between oocytes and the companion granulosa cells is essential for the development and functions of both compartments. Oocytes are deficient in their ability to transport certain amino acids and in carrying out glycolysis and cholesterol biosynthesis. Cumulus cells must provide them with the specific amino acids and the products in these metabolic pathways. Oocytes c...

BACKGROUND Dialogue between the oocyte and cumulus cells is essential for oocyte maturation. A prospective laboratory research project was designed to evaluate transcription of specific genes in cumulus cells harvested before intracytoplasmic sperm injection from pre-ovulatory follicles, according to individual oocyte nuclear maturity and developmental competence. Genes were chosen because thei...

In mammals, ovulation requires a tight control of extracellular matrix modifications, within both the follicle wall and the inner mass of granulosa cells surrounding the oocyte, namely the cumulus cells. During the pre-ovulatory period, mural granulosa cells promote selective degradation of perifollicular matrix, resulting in the formation of a follicle rupture site. Conversely, cumulus cells s...

Background: The buffalo (Bubalus bubalis) is the animal with special production characteristics in producing milk and meat has a critical role in rural industry in Iran. The most problem of this specie is its low reproductive output. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on maturation rate of Buffalo oocyte.Materials and Methods: Ovaries were colle...

Glucose is an essential nutrient for mammalian cells. Emerging evidence suggests that glucose within the oocyte regulates meiotic maturation. However, it remains controversial as to whether, and if so how, glucose enters oocytes within cumulus-oocyte complexes (COCs). We used a fluorescent glucose derivative (6-NBDG) to trace glucose transport within live mouse COCs and employed inhibitors of g...

We previously reported that when midkine (MK), a heparin-binding growth differentiation factor was used in in vitro maturation (IVM) culture of bovine cumulus-enclosed oocytes (CEOs), their developmental competence to the blastocyst stage after in vitro fertilization (IVF) was enhanced and the effect of MK might be mediated by its action upon mural granulosa cells and cumulus cells that closely...

To acquiring high-quality embryos is crucial to the improvement of pregnancy rates for assisted reproductive technology (ART). But how to find a non-invasive approach to early embryo selection and improve the implantation rates from proteins in cumulus cells? Our previously study showed that acetyl-CoA acyltransferase 2 (ACAA2) protein and adenine-thymine (AT)-rich interactive domain-containing...