The gene for the FeS protein of the Rhodopseudomonas sphaeroides b/c1 complex was identified by means of cross-hybridization with a segment of the gene encoding the corresponding FeS protein of Neurospora crassa. Plasmids (pRSF1-14) containing the cross-hybridizing region, covering in total 13.5 kb of chromosomal DNA, were expressed in vitro in a homologous system. One RSF plasmid directed the ...

Numerous attempts have been made to separate the insoluble cytochromes of mammalian heart muscle. Von Euler and Hellstriim in 1939 (1) used sodium cholate and ammonium sulfate and obtained a preparation containing only cytochrome b. Straub (2) obtained a fraction with sodium cholate and ammonium sulfate in which he demonstrated cytochrome oxidase (called cytochrome a3 by him after Keilin and Ha...

In chloroplasts, binding of a c'-heme to cytochrome b(6) on the stromal side of the thylakoid membranes requires a specific mechanism distinct from the one at work for c-heme binding to cytochromes f and c(6) on the lumenal side of membranes. Here, we show that the major protein components of this pathway, the CCBs, are bona fide transmembrane proteins. We demonstrate their association in a ser...

Sumatran elephant (Elephas maximus sumatranus Temminck, 1847) is one of the Asian sub-species distributed in Sumatera Island and classified as endangered species due to hunting high habitat fragmentation. This study aimed analyze genetic relationship elephants based on mitochondrial cytochrome b gene (Cyt b). Blood samples were collected from 11 Seblat Natural Ecotourism Park. Based mt-DNA Cyto...

The lumen segment of cytochrome f consists of a small and a large domain. The role of the small domain in the biogenesis and stability of the cytochrome b(6)f complex and electron transfer through the cytochrome b(6)f complex was studied with a small domain deletion mutant in Chlamydomonas reinhardtii. The mutant is able to grow photoautotrophically but with a slower rate than the wild type str...

In the stroma compartment, several pathways are used for integration/translocation of chloroplast proteins into or across the thylakoid membrane. In this study we investigated the mode of incorporation of the chloroplast-encoded cytochrome b(6) into the bacterial membrane. Cytochrome b(6) naturally comprises of four transmembrane helices (A,B,C,D) and contains two b-type hemes. In the present s...

White, David C. (University of Kentucky College of Medicine, Lexington). Synthesis of 2-demethyl vitamin K(2) and the cytochrome system in Haemophilus. J. Bacteriol. 89:299-305. 1965.-The synthesis of the respiratory quinone, 2-demethyl vitamin K(2), is stimulated in Haemophilus parainfluenzae under conditions which provoke the synthesis of the cytochrome system. However, the various components...

Irradiation of cells of Prototheca zopfii with blue light inhibited the respiratory capacity of the cells. The inhibition of respiration was correlated with a photodestruction of cytochrome c(551), cytochrome b(559), and cytochrome a(3). Cytochrome c(549), cytochrome b(555), and cytochrome b(564) were unaffected by the irradiation treatment. The alpha-band of reduced cytochrome a was shifted fr...

The dissociation constants for the binding of Rhodobacter capsulatus cytochrome c2 and its K93P mutant to the cytochrome bc1 complex embedded in a phospholipid bilayer were measured by plasmon waveguide resonance spectroscopy in the presence and absence of the inhibitor stigmatellin. The reduced form of cytochrome c2 strongly binds to reduced cytochrome bc1 (Kd = 0.02 microM) but binds much mor...

The cytochrome composition of 19 strains of Actinoplanaceae has been investigated. The principal cytochrome found was cytochrome b, and occasional strains showed the presence of cytochrome a.