The effect of differentiation induction by a tumor-promoting phorbol diester. 1 2-O-tetradecanoylphorbol-1 3-acetate (TPA) on a clonal human megakaryoblastic cell line. MEG01 s. was investigated. and a prominent response was demonstrated. The cells became weakly adherent, developed conspicuous cytoplasmic blebs. and displayed mature megakaryocytic characteristics by light microscopy such as the...

Ultrastructural evaluation of eosinophilic leukocytes from a 2-yr-old asymptomatic girl with chronic benign neutropenia (CBN) revealed a variety of morphological abnormalities. All eosinophils obtained from blood and marrow specimens contained multipole microcrystalloids in most of the mature cytoplasmic granules. An increase in crystalloid-free, immature granules in late (bilobed nuclei) eosin...

Nuage, a germ line specific organelle, is remarkably conserved between species, suggesting that it has an important germline cell function. Very little is known about the specific role of this organelle, but in Drosophila three nuage components have been identified, the Vasa, Tudor and Aubergine proteins. Each of these components is also present in polar granules, structures that are assembled ...

The effect of differentiation induction by a tumor-promoting phorbol diester. 1 2-O-tetradecanoylphorbol-1 3-acetate (TPA) on a clonal human megakaryoblastic cell line. MEG01 s. was investigated. and a prominent response was demonstrated. The cells became weakly adherent, developed conspicuous cytoplasmic blebs. and displayed mature megakaryocytic characteristics by light microscopy such as the...

Since pinocytosis has only been recently recognized in polymorphonuclear leukocytes (PMNs), little is known about the fate of pinosomes. Here we report that pinosomes can fuse with the cytoplasmic granules of PMNs. We also find that at least for a short period of time after formation, pinosomes can fuse with the plasma membrane and release their contents to the outside. We present a morphologic...

Background and AimsStress granules (SGs) as membrane-less cytoplasmic foci formed in response to unfavorable external stimuli could promote cancer cells adapt hostile environments. Hepatocellular carcinoma (HCC) is prone be highly aggressive once diagnosed, which markedly reduces patient survival time. Therefore, it crucial develop valid diagnostic markers prognosticate HCC prognosis, promotes ...

A female DBA/2 mouse is characterized by the presence of abundant cytoplasmic granules in the renal tubules. In the present study, the morphometrics of kidneys from female DBA/2 mice at 5, 15, 18, 21, and 24 months of age were investigated to determine the age-dependent renal changes in this mouse strain. Glomerular and tubulointerstitial disease progressed with age, and the semiquantitative sc...

Activation of human neutrophils involves the degranulation of specific and azurophil granules. This process is GTP-dependent and the presence of small GTP-binding proteins (SGBPs) has been detected in the two granule populations. At present, none of these SGBPs has been definitely identified. In order to characterize some of these proteins and obtain further insights as to their potential role ...

A49 year old woman presented with a lump in her right breast, which was partially cystic, and after draining refilled immediately. A biopsy showed an infiltrating ductal carcinoma grade 3, with the additional feature of unusual cytoplasmic granules. Several courses of cytotoxic chemotherapy were administered which included adriamycin, cyclophosphamide, methotrexate, and 5 fluorouracil. Eight mo...