Cell apoptosis refers to the active death process of cells controlled by genes for multi-cellular organisms to regulate development and maintain homeostasis. It is one of the main means of senescence and natural death of cells, and is necessary for organisms to maintain normal physiological processes. Interest in apoptosis has increased due to the recognition that many diseases involve too much...

A series of comparative studies on the chemical and biological characterizations of the ovine lutropin subunits and their recombinants prepared by a countercurrent distribution (CCD) procedure and a salt precipitation method is presented. The salt precipitation Method B (0.1 M acetate and 3 M NaCl) gave a low yield of pure subunits and the subunits thus prepared recombined poorly to form a prod...

Functionalized phenols based on tyramine were synthesized in order to be selectively grafted on to hexachlorocyclotriphosphazene, affording a variety of functionalized dendrons of type AB(5). The B functions comprised fluorescent groups (dansyl) or dyes (dabsyl), whereas the A function was provided by either an aldehyde or an amine. The characterization of these dendrons is reported. An unexpec...

A sensitive method is described for the detection of glycoproteins after polyacrylamide gel electrophoresis. Dansyl hydrazine is condensed with aldehydic functions generated by periodate oxidation of protein bound carbohydrate. The resulting hydrazones are reduced to the stable hydrazine derivatives with sodium borohydride. After destaining, the fluorescent-labeled glycoproteins are visualized ...

Pseudomonas cepacia was found to be resistant to the outer membrane-permeabilizing effects of aminoglycoside antibiotics, polymyxin B, and EDTA. Permeabilization of P. cepacia to the fluorescent probe 1-N-phenylnaphthylamine was not achieved at concentrations 100- to 1,000-fold above those required to permeabilize Pseudomonas aeruginosa. Furthermore, in contrast to P. aeruginosa cells, intact c...

The effects of non-ionic surfactants as mobile phase additives on the fluorescence intensity of dansyl derivatives of biogenic amines in high-performance thin-layer chromatography (HPTLC) were investigated. The dansyl derivatives of putrescine, cadaverine, spermidine and spermine were separated by HPTLC with silica gel as stationary phase and an eluent consisting of chloroform–triethylamine (2 ...

Dansylated analogues of the potent and selective micro opioid peptide agonist [Dmt(1)]DALDA (H-Dmt-D-Arg-Phe-Lys-NH(2); Dmt = 2',6'-dimethyltyrosine) were prepared either by substitution of N(beta)-dansyl-alpha,beta-diaminopropionic acid or N(epsilon)-dansyllysine for Lys(4), or by attachment of a dansyl group to the C-terminal carboxamide function via a linker. All three analogues displayed hi...

Steroid concentrations in serum are fluctuating during pregnancy of many mammal species. The current knowledge about endocrinology gestation is mainly based on immunoassays. However, the lack specificity these assays hampers reliability results. In present work, we developed and validated a methodology associating liquid chromatography (LC) mass spectrometry (MS) to simultaneously quantify, wit...