This paper describes a rapid two-step procedure for the purification of the low density lipoprotein receptor from bovine adrenal cortex membranes. After solubilization with nonionic detergents, the receptor adheres tightly to a DEAE-cellulose column at pH 6. Following elution from DEAE-cellulose, detergent is removed, leaving the receptor in a soluble form. The receptor is then subjected to aff...

The major peak of radioactivity from liver extracts appears to behave as a folate polyglutamate on Sephadex G-15, but its behaviour on DEAE cellulose is not consistent with a folate polyglutamate. The second, smaller peak behaved as a monoglutamate on both Sephadex G-15 and DEAE-cellulose, but could not be matched with any authentic folate monoglutamate. The present results fail to provide evid...

1. Cell-free lysates of human peripheral blood lymphocytes contained two casein kinase activities and two histone kinase activities, which could be separated by chromatography on DEAE-Sephadex. 2. Neither of the casein kinase activities were stimulated by cyclic AMP. The major activity was eluted from DEAE-Sephadex between 0.4 and 0.45M-KCI, had a molecular weight of approx. 130000 (sucrose den...

The present study outlines the synthesis of a new microcarrier for anchoragedependent animal cell cultures similar to Cytodex 1. The new microcarriers were synthesized from the cross-linking soybean starch microspheres followed by modification with 2-diethylaminoethyl (DEAE). DEAE–soybean starch microspheres were used in anchorage-dependent cell cultures using 3 g/L of the carrier and a 2.0 × 1...

We compared growth of the recently discovered respiratory pathogen Chlamydia pneumoniae in McCoy, HeLa 229, BHK-21, and HL cells. When cells were not pretreated with DEAE-dextran, HL cells had significantly higher mean numbers of inclusion-forming units (IFUs) on initial inoculation than the other cell lines. When cells were pretreated with DEAE-dextran, HeLa 229 and HL cells had equivalent mea...

Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange columns. L. Kansas virus eluted from DEAE columns showed one peak of hemagglutin...

Several reports have appeared that suggest that 24,25-dihydroxycholecalciferol has a possible biological role in bone formation. We have utilized competition studies, saturation analysis, sucrose-density-gradient sedimentation and DEAE-cellulose chromatography to demonstrate that long bones of vitamin D-depleted newborn rats contain cytoplasmic and possibly nuclear receptors that bind 24,25-dih...

* To whom correspondence should be addrcsscd. Step 2・ . To 61 of homogenate, DEAE-cel]ulose (600g, wet wci.oht) pre-equilibruted with 10mM sodium acctate buffer (pH 6,O) was added and thc suspension was stirred gent]y at O"C fbr 3emin, The DEAE-cellu]ose-udsorbcd protein was coilected by suction and washed with 3 1 of thc same bufi'er. then suspendcd in the same buffcr. The column packcd -・ith ...