The contribution of free amino acids and thiamine to the production potent meat aroma compounds in nitrite-reduced, dry-fermented sausages inoculated with a D. hansenii strain was objective this study. For this, three different sausage formulations were manufactured; control two reduced by half nitrate nitrite one them hansenii. Free acids, content savoury volatile analysed. Eleven quantitated....

Several aspects concerning the bioconversion of xylose-containing hydrolysates (obtained from Eucalyptus wood) into xylitol were assessed. Debaryomyces hansenii yeast strains were adapted to fermentation media (obtained either by prehydrolysis or autohydrolysis-posthydrolysis of wood) supplemented with low-cost nutrients. Media containing up to 80 g/L xylose were efficiently fermented when the ...

DNA microarrays of 86 genes from the yeasts Debaryomyces hansenii, Kluyveromyces marxianus, and Yarrowia lipolytica were developed to determine which genes were expressed in a medium mimicking a cheese-ripening environment. These genes were selected for potential involvement in lactose/lactate catabolism and the biosynthesis of sulfur-flavored compounds. Hybridization conditions to follow speci...

A prolyl aminopeptidase (PAP) (EC 3.4.11.5) was isolated from the cell extract of Debaryomyces hansenii CECT12487. The enzyme was purified by selective fractionation with protamine and ammonium sulfate, followed by two chromatography steps, which included gel filtration and anion-exchange chromatography. The PAP was purified 248-fold, with a recovery yield of 1.4%. The enzyme was active in a br...

Under non-phosphorylating conditions a high proton transmembrane gradient inhibits the rate of oxygen consumption mediated by the mitochondrial respiratory chain (state IV). Slow electron transit leads to production of reactive oxygen species (ROS) capable of participating in deleterious side reactions. In order to avoid overproducing ROS, mitochondria maintain a high rate of O(2) consumption b...

Background Cellulose is a homopolysaccharide composed of extracellular D-glucose monomers connected by glycosidic linkages in b-1,4 conformation which can be synthesized by a variety of living organisms, possessing numerous applications in food, pharmaceutical, medical area, etc.[1][2]. In 1990, cellulose synthase operon encoding four proteins required for bacterial cellulose (BC) biosynthesis ...