The gene of GRA6 of Toxoplasma gondii pig strain was cloned; and the value of recombinant GRA6 as a diagnostic antigen of toxoplasmosis in China was investigated. A pair of primers was synthesized according to the DNA sequence of GRA6 of T. gondii RH strain, and the DNA of pig strain tachyzoites was prepared. A single, specific DNA fragment was successfully amplified from the genomic DNA of tac...

Toxoplasma gondii is a ubiquitous, unicellular, eukaryotic parasite with a complex intracellular life cycle capable of invading and chronically infecting a wide variety of vertebrate host species, including man. Although normally opportunistic in healthy adults, it is a lethal pathogen in immunocompromised humans, particularly in AIDS patients. We present the application of a genomic phage disp...

Horse eosinophil function has been studied in vitro by means of phase contrast cinemicrophotography. Locomotion of horse eosinophils was inhibited by serum factors reacting with glass surfaces. Under appropriate conditions which eliminated this inhibitory effect, eosinophils moved about and ingested some particles as rapidly as did neutrophils. Eosinophils were attracted to and readily engulfed...

Using a new technique for antigen localization. we have demonstrated platelet proteins in megakaryocytes in plastic-embedded biopsy specimens of normal human bone marrow. In a series of 25 specimens. megakaryocytes showed labeling with antibodies to the integral membrane glycoproteins lIla. llb. and the lIb-Illa complex; granule membrane protein 140; and five alpha-granule matrix proteins: thro...

Using a new technique for antigen localization. we have demonstrated platelet proteins in megakaryocytes in plastic-embedded biopsy specimens of normal human bone marrow. In a series of 25 specimens. megakaryocytes showed labeling with antibodies to the integral membrane glycoproteins lIla. llb. and the lIb-Illa complex; granule membrane protein 140; and five alpha-granule matrix proteins: thro...

The involvement of metabolites of arachidonic acid in platelet-dense granule secretion and secondary platelet-platelet interactions is well characterized. However, their role in heterotypic interactions dependent on alpha-granule secretion is less well understood. Using platelet-surface expression of P-selectin as a marker of alpha-granule secretion, we have shown that: (1) aspirin treatment of...

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The clinical manifestations of platelet dense ( delta ) granule defects are easy bruising, as well as epistaxis and bleeding after delivery, tooth extractions and surgical procedures. The observed symptoms may be explained either by a decreased number of granules or by a defect in the uptake/release of granule contents. We have developed a method to study platelet dense granule storage and rele...

After implantation into the external germinal layer of early postnatal cerebellum, primary external germinal layer progenitor cells gave rise exclusively to granule neurons. In contrast, all major classes of cerebellar cells were observed following implantation of embryonic day 13 cerebellar precursor cells into the external germinal layer. These results suggest that granule cells arise from pr...

Identifying the mechanisms underlying the contribution of coagulation factor XII (fXII) to arterial thrombosis is of particular clinical interest. The physiological importance of platelet dense granules-derived polyphosphates in fXII activation is a subject of intense discussion and controversy. Our previous study suggested that the potently activated platelet-surface plays the more essential r...