A DNA sequence polymorphism, revealed by digestion of human DNA with the restriction endonuclease Sst-1 and hybridization with an apolipoprotein A-I complementary DNA clone, has been shown to be located in or close to the 3' noncoding region of the apolipoprotein C-III gene. This polymorphism is found in significantly increased prevalence (P < 0.001) in Caucasian hypertriglyceridemic subjects c...

The forensic biologist has traditionally used a large set of markers including blood group antigens and serum proteins to establish links between evidence and comparison samples. These markers are polymorphic and genetically determined. In 1985, a new technique was introduced which opened the door to the forensic use of deoxyribonucleic acid (DNA) technology: its inventor coined the term ‘DNA R...

When bacteriophage SP02 infects a Bacillus subtilis culture during or shortly after transformation, the frequency of transformants among the resulting lysogens is greatly reduced relative to that in the uninfected culture. The effect can occur after the deoxyribonucleic acid has been taken up and covalently attached to recipient deoxyribonucleic acid.

In a study on Feulgen hydrolysis of frozen-dried alcohol-fixed lily anthers, a chromatographic technique was developed to analyze the acid hydrolysate for some of the degradation products of nucleic acid. Hydrolysis was accomplished by 10 per cent perchloric acid at 20 degrees C., and a typical hydrolysis time-Feulgen intensity curve was obtained, with maximum staining occurring at 19 hours. Mi...

microarray data have an important role in identification and classification of the cancer tissues. having a few samples of microarrays in cancer researches is always one of the most concerns which lead to some problems in designing the classifiers. for this matter, preprocessing gene selection techniques should be utilized before classification to remove the noninformative genes from the microa...

Although several methods (1-5) are used for the determination of deoxyribonucleic acid, none is without some limitations. For example, procedures (1) that depend on the analysis of phosphate in fractionated materials are of limited sensitivity. The use of the absorption at 260 rnp as a measure of deoxyribonucleic acid (2) does not provide a procedure giving readily comparable values for differe...

Human gene therapy is the introduction of new genetic material into the cells of an individual with the intention of producing a therapeutic benefit for the patient. Deoxyribonucleic acid and ribonucleic acid are used in gene therapy. Over time and with proper oversight, human gene therapy might become an effective weapon in modern medicine's arsenal to help fight diseases such as cancer, acqui...

Studies on deoxyribonucleic acid purified from the granulosis virus of Trichoplusia ni have revealed the presence of a closed, double-stranded superhelix which sediments at 95S relative to relaxed circles (74S) and linear (60S) forms. Molecular weight estimates show that this insect virus deoxyribonucleic acid has a size of 100 x 10(6) daltons.