OBJECTIVE To evaluate the efficacy of NESTROFT (Naked Eye Single Tube Red Cell Osmotic Fragility Test) as a screening tool for detection of beta thalassemia trait. DESIGN Prospective study. SETTING Field camps in various parts of Gujarat and Maharashtra States. METHODS A total of 2525 subjects were screened. NESTROFT, complete hemogram including red cell indices and calculation of Mentzer...

Light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case. Live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongated shapes. When moving, amoebae formed cytoplasmic projections such as wide lamellae and acanthopo...

Mucins play pivotal roles in influencing cancer biology, for example affecting carcinoma invasion, aggressiveness and/or metastatic potential. Our aim is to investigate the significance of expression profiles of two mucins in particular, MUC1 and MUC2, their correlations with various clinicopathological features, and prognosis in gallbladder adenocarcinoma (GBAC). We performed immunohistochemis...

The rainfall amount and the frequency of extreme events have been predicted to increase in Qinghai-Tibetan Plateau during growing season. These changes will likely affect ecosystem processes, including those that control nitrogen (N) cycling storage; however, direction remains unclear. In this study, we experimentally altered precipitation season (May through October) at an alpine wetland Plate...

IgE antibodies bound to cell-surface receptors, Fc RI, crosslink through the binding of antigens on the cell surface. This formation of aggregates is what stimulates mast cells and basophils in order to initiate degranulation, resulting in an allergic response. Nearly 1,500 Americans die each year from anaphylactic shock predicated by aggregation. Experimental studies have shown the spatial org...

Pancreatic cancer mucins have several carbohydrate antigens that are potentially useful in the detection of pancreatic cancers, but little is known about the core polypeptides of pancreatic cancer mucins. In this study, purified mucin from SW1990 pancreatic cancer xenografts was deglycosylated by treatment with hydrogen fluoride to give pancreatic cancer apomucin. Consistent with near-complete ...

The stink bug Dichelops furcatus (F.) (Heteroptera: Pentatomidae) has increased in abundance in recent years on the wheat, Triticum aestivum L., crop cultivated in the southern region of Brazil. To investigate the probing (stylet penetration) behaviors and nonprobing behaviors of D. furcatus on wheat plants, the electrical penetration graph or electropenetrography (EPG) technique was applied. N...

background: acanthamoeba spp. is the causative agent of blindness keratitis and fatal encephalaitis. presence of acanthamoeba spp. in a wide variety of niches such as different water types can lead to exposure of high risk people such as contact lens wearers. the main aim of the present study was to explore the occurrence of acanthamoeba genotypes in the recreational water sources using both mo...