1. The dealkylation of the insecticidal phosphoric acid triester, 2-chloro-1-(2,4-dichlorophenyl)vinyl diethyl phosphate, proceeds in mammalian liver slices via an oxidative mechanism and not by hydrolysis. 2. The enzyme that catalyses the reaction is located in the microsomal fraction of liver homogenate and is dependent for activity on molecular oxygen and NADPH. 3. There are large species di...

Abstract: Aim: To investigate chemo-preventive effect of methanolic fruit extract Cucumis melo var. agrestis on DEN (diethyl-nitrosamine) induced hepatocellular carcinoma in Sprague Dawley rats. Materials and Methods: Five groups rats randomly selected comprising 6 animals each group, all groups, except Group-1 were administered single dose (200 mg/kg, i.p. dissolved PBS) for the induction HCC ...

The externally controlled cleavage of covalently linked prodrugs, proteins, or solid-phase formulation vehicles offers potential advantages for controlled drug or gene delivery. A series of o-nitrobenzyl ester compounds (1-8) were synthesized to allow a systematic study of photolability. The o-nitrobenzyl ester was strictly required for photolability, while imido esters were not photolabile. Th...

Trace levels of amoxicillin residues were determined in animal tissues by liquid chromatography (LC) with fluorescence detection. An improved solid-phase extraction (SPE) procedure requiring less flammable solvent (diethyl ether) was developed for sample preparation. Muscle samples of beef, pork, chicken, and tilapia were extracted with a phosphate buffer followed by the modified SPE procedure ...

Tyrosine phenol-lyase was purified 32-fold from Aeromonas phenologenes ATCC 29063, the organism that produces phenol in refrigerated haddock. The purification procedure included ammonium sulfate fractionation, protamine sulfate treatment, and column chromatography with Sephadex G-200, diethyl-aminoethyl-cellulose, and hydroxyapatite. The enzyme was found to be thermally inactivated at temperatu...

An enzyme capable of hydrolyzing 4-methylumbelliferyl phenylphosphonate to 4-methylumbelliferone and phenylphosphonic acid has been detected in human serum. It has a Km value of 1.72 x 10(-4) mol/L, has an optimum pH of 8.8-9.1 in Tris buffer, and shows maximum activity at 60 degrees C (30 min). The enzymic activity can be inhibited by Na3PO4, EDTA, and cysteine. We saw no effect of CuSO4, aden...

The conversion of ethanol towards ethylene and diethyl ether in the presence catalysts requires special consideration from perspective green chemistry. Ethanol dehydration was studied on a complex titanium phosphate MAlTiP (M0.5(1+x)AlxTi2-x(PO4)3 with M = Ni, Mn (x 0; 0.2)) catalysts, alongside NASICON-type structure synthesized by sol–gel method. initial were characterized N2 gas sorption, SE...

background: cold-induced aggregation micro extraction based (ciame) based on ionic liquid was used as a rapid and simple method for determination trace amounts of cadmium and lead in milk powder by analysis with flame atomic absorption spectrometry (faas). methods: sample solution containing of cd2+ and pb2+ in dynamic range, phosphate buffer (ph = 9, 3 ml), diethyl dithiocarbamate (complexing ...