Often plant tissues are recalcitrant and, due to that, methods relying on protein precipitation, such as TCA/acetone precipitation and phenol extraction, are usually the methods of choice for protein extraction in plant proteomic studies. However, the addition of precipitation steps to protein extraction methods may negatively impact protein recovery, due to problems associated with protein re-...

Acemannan from Aloe barbadensis was obtained with four processes: 1) size exclusion chromatography (SEC) using Sepharose CL-4B matrix followed by ethanolic precipitation; 2) SEC, ultrafiltration using hollow-fiber cartridges (30 kDa or 0.1 μm) and ethanolic precipitation; 3) SEC, precipitation with cetyltrimethylammonium bromide (CTAB) and ethanolic precipitation; and 4) direct precipitation wi...

Extreme weather events such as precipitation extremes and severe storms cause hundreds of deaths and injuries annually in the United States. Climate change may alter the frequency, timing, intensity, and duration of these events. Increases in heavy precipitation have occurred over the past century. Future climate scenarios show likely increases in the frequency of extreme precipitation events, ...

HDL consists of two major subfractions, HDL2 and HDL3. This paper describes a simple method for assaying HDL subspecies by combining a single precipitation with a direct high density lipoprotein-cholesterol (HDL-C) assay. A precipitation reagent (0.06 ml) containing 1,071 U/ml heparin, 500 mmol/l MnCl2) and 12 mg/ml dextran sulfate was added to a serum (0.3 ml). The sample was incubated and cen...

This paper investigates the benefits of integrating coarse resolution satellite-derived precipitation estimates with quasi-point rain gauge data for generating a fine spatial resolution precipitation map product. To integrate the two precipitation data sources, a geostatistical downscaling and integration approach is presented that can account for the differences in spatial resolution between d...

We investigated the large-scale connection between African aerosol and precipitation in the West African Monsoon (WAM) region using 8-year (2000–2007) monthly and daily Moderate Resolution Imaging Spectroradiometer (MODIS) aerosol products (aerosol optical depth, fine mode fraction) and Tropical Rainfall Measuring Mission (TRMM) precipitation and rain type. These high-quality data further confi...

The ongoing changes in vegetation spring phenology in temperate/cold regions are widely attributed to temperature. However, in arid/semiarid ecosystems, the correlation between spring temperature and phenology is much less clear. We test the hypothesis that precipitation plays an important role in the temperature dependency of phenology in arid/semiarid regions. We therefore investigated the in...