De novo enzymes can be created by computational design and directed evolution. Here, we review recent insights into the origins of catalytic power in evolved designer to pinpoint opportunities for next-generation designs: Evolution precisely organizes active sites, introduces H-bonding networks, invokes electrostatic catalysis, creates dynamical networks embedding site a reactive protein scaffo...

INTRODUCTION Stereoselective chemical reactions have become extremely important in both academic and industrial settings for the synthesis of useful molecules. Catalytic reactions are of particular importance due to their capacity to effect otherwise impossible transformations using substoichiometric quantities of the catalyst. Traditionally, transition metal catalysts have played a central rol...

Fungal laccases are generalists biocatalysts with potential applications that range from bioremediation to novel green processes. Fuelled by molecular oxygen, these enzymes can act on dozens of molecules of different chemical nature, and with the help of redox mediators, their spectrum of oxidizable substrates is further pushed towards xenobiotic compounds (pesticides, industrial dyes, PAHs), b...