BACKGROUND A simple method for the measurement of LDL particle sizes is needed in clinical laboratories because a predominance of small, dense LDL (sd LDL) has been associated with coronary heart disease. We applied dynamic light scattering (DLS) to measure lipoprotein particle sizes, with special reference to sd LDL. METHODS Human serum lipoproteins isolated by a combination of ultracentrifu...

THE problem of the isolation of cell components in quantity has been approached in several ways. For the nucleus the methods have included acid-pepsin digestion of the cytoplasm (22), hemolysis of nucleated erythrocytes (1, 33, 19), and recently the differential centrifugation of mechanically disrupted cells. In preparing tissues for differential centrifugation the following procedures have bee...

in this article differential transformation method (dtms) has been used to solve neutral functional-differential equations with proportional delays. the method can simply be applied to many linear and nonlinear problems and is capable of reducing the size of computational work while still providing the series solution with fast convergence rate. exact solutions can also be obtained from the kno...

OBJECTIVE develop a radiographic procedure to measure dorsolateral subluxation (DLS) of the femoral head in canine coxofemoral (hip) joints in a weight-bearing position. STUDY DESIGN DLS measured on a radiographic projection was compared with DLS measured on computed tomography (CT) images of hip joints in a weight-bearing position. ANIMALS A total of 24 dogs of varying ages were examined i...

Biocompatible polymeric materials with potential to form functional structures in association with different therapeutic molecules have a high potential for biological, medical and pharmaceutical applications. Therefore, the capability of the inclusion of nano-Complex formed between the sodium salt of poly(maleic acid-alt-octadecene) and a β-lactam drug (ampicillin trihydrate) to avoid the chem...

Biocompatible polymeric materials with potential to form functional structures in association with different therapeutic molecules have a high potential for biological, medical and pharmaceutical applications. Therefore, the protective capability of the inclusion nano-Complex formed between the sodium salt of poly(maleic acid-alt-octadecene) and a β-lactam drug (ampicillin trihydrate) on the ch...

Extracellular vesicles (EVs) are membrane enclosed (<1 µm), such as exosomes (30-150 nm), involved in cell communication, which have important biological implications. In this study, EV preparations were enriched for from human serum by polyethylene glycol (PEG) precipitation. Different variables of the PEG precipitation method (i.e. concentration PEG, filtration and centrifugation resuspended ...

1. A method is described whereby the major components of liver suspensions are segregated according to size into three main fractions: (a) a large granule fraction composed of elements approximately 0.5 to 2 micro in diameter; (b) a microsome fraction composed of submicroscopic elements approximately 80 to 150 mmicro in diameter; and, (c) a supernate fraction containing the soluble components o...