Infertility affects 15% of all couples. Although male infertility factors with reduced semen quality are contributing to about half of all involuntary childlessness, the value of standard semen parameters in prediction of fertility in vivo and choice of proper method for assisted reproduction is limited. In the search for better markers of male fertility, during the last 10 years, assessment of...

BACKGROUND The sperm chromatin structure assay (SCSA) provides an objective assessment of sperm chromatin integrity, which is essential for normal sperm function. SCSA is valuable as a fertility marker in epidemiological studies and in the clinical situation. Little is known about the impact of testicular and post-testicular function on SCSA parameters. METHODS Ejaculates from 278 military co...

OBJECTIVE To examine the relationship among sperm apoptosis, sperm chromatin status, and DNA ploidy in different sperm fractions. DESIGN Prospective study. SETTING Reproductive research center in a tertiary care hospital. INTERVENTION(S) Sperm prepared by density gradient were evaluated for sperm count, motility, apoptosis, and sperm chromatin assessment. MAIN OUTCOME MEASURE(S) Sperm c...

OBJECTIVE To examine levels of sperm DNA damage and oxidative stress (OS) in infertile men with varicocele. DESIGN Prospective controlled study. SETTING Male infertility clinic, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio. PATIENT(S) Thirty-one infertility patients and 16 fertile controls. INTERVENTION(S) Sperm DNA fragmentation index (DFI), levels of sem...

In this research, post-thaw effects of bull semen extenders including different amounts of boron (sodium pentaborate) on total motility (TMOT), progressive motility (PMOT), kinetic parameters of spermatozoa (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plasma membrane and acrosome integrity (PMAI) and DNA fragmentation index (DFI) were evaluated. Eight ejaculates were examined from four different b...

In this study, the complexity of chromatin integrity was investigated in frozen-thawed semen samples from 37 sires with contrasting fertility, expressed as 56-day non-return rates (NR56). Protamine deficiency, thiols, and disulfide bonds were assessed compared previously published data for DNA fragmentation index (DFI) high stainability (HDS). addition, vitro embryo development sperm methylatio...

Background: Are dietary patterns, substantiated by biomarkers, associated with semen quality? Materials and Methods: In 161 men of subfertile couples undergoing in vitro fertilization treatment in a tertiary referral clinic in Rotterdam, the Netherlands, we assessed nutrient intakes and performed principal component factor analysis to identify dietary patterns. Total homocysteine (tHcy), folate...

Objectives. To evaluate the sperm chromatin dispersion in men with infertility using first Russian kit. Methods. The study was conducted between July and August 2022. Study participants were 492 male volunteers aged 23 to 59 years which semen samples examined by GEMSTANDART–HaloSperm L&Q kit so-called Halo test. outcomes as a percentage DNA Fragmentation Index (DFI), %. results of test eval...

One of the most significant indicators affecting male fertility is sperm nuclear and mitochondrial DNA fragmentation index (DFI). damage depends on biotic abiotic factors, leading to oxidative stress (O.S.). This research aimed investigate relationship between dysfunction spermatozoa their biological adequacy. The material was frozen-thawed samples from Ayrshire, Russian Black Pied Holstein, Re...