نتایج جستجو برای: dna immunization

تعداد نتایج: 538548  

Journal: :Journal of Investigative Dermatology 2022

Skin is a unique target for vaccination. Targeting keratinocytes to produce proinflammatory mediators improve immunity rational approach vaccine design. Keratinocytes genetically engineered overexpress the stress response related transcription factor x-box binding protein 1 (XBP1) increased production of and co-delivered antigen. Keratinocyte-specific overexpression XBP1 through cutaneous genet...

ژورنال: :jundishapur journal of microbiology 0
fatemeh tabatabaie department of parasitology and mycology, school of medicine, iran university of medical sciences (iums), tehran, ir iran mehdi mahdavi department of virology, pasteur institute of iran, tehran, ir iran sobhan faezi department of bacteriology, pasteur institute of iran, tehran, ir iran abdolhossein dalimi department of parasitology, faculty of medical sciences, tarbiat modares university, tehran, ir iran zohreh sharifi blood transfusion research center, high institute for research and education in transfusion medicine, tehran, ir iran lame akhlaghi department of parasitology and mycology, school of medicine, iran university of medical sciences (iums), tehran, ir iran

background: the thiol-specific antioxidant (tsa) is an antigen of leishmania major which is believed to be the most promising molecule as a vaccine candidate against leishmaniasis. objectives: in this study, we investigated the protective efficacy of tsa-based dna vaccine against l. major infection. materials and methods: recombinant plasmid construction tsa (pctsa) was prepared and transfected...

Journal: :Journal of virology 2003
John R Mascola Mark G Lewis Thomas C VanCott Gabriela Stiegler Hermann Katinger Michael Seaman Kristin Beaudry Dan H Barouch Birgit Korioth-Schmitz Georgia Krivulka Anna Sambor Brent Welcher Daniel C Douek David C Montefiori John W Shiver Pascal Poignard Dennis R Burton Norman L Letvin

High levels of infused anti-human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibodies (MAbs) can completely protect macaque monkeys against mucosal chimeric simian-human immunodeficiency virus (SHIV) infection. Antibody levels below the protective threshold do not prevent infection but can substantially reduce plasma viremia. To assess if HIV-1/SIV-specific cellular immunit...

Journal: :hepatitis monthly 0
leila pishraft sabet department of virology, school of public health, tehran university of medical sciences, tehran, ir iran; razi vaccine and serum research institute, karaj, ir iran tahereh taheri molecular immunology and vaccine research laboratory, pasteur institute of iran, tehran, ir iran arash memarnejadian hepatitis and hiv laboratory, pasteur institute of iran, tehran, ir iran talat mokhtari azad department of virology, school of public health, tehran university of medical sciences, tehran, ir iran fatemeh asgari department of virology, school of public health, tehran university of medical sciences, tehran, ir iran ramin rahimnia department of nano medicine, school of advanced technologies in medicine, tehran, ir iran

background hypervariability of hcv proteins is an important obstacle to design an efficient vaccine for hcv infection. multi-epitope vaccines containing conserved epitopes of the virus could be a promising approach for protection against hcv. objectives cellular and humoral immune responses against multi-epitope dna and peptide vaccines were evaluated in balb/c mice. materials and methods in th...

1999
Carlos Chagas Filho

The induction of systemic (IgG) and mucosal (IgA) antibody responses against the colonization factor I antigen (CFA/I) of enterotoxigenic Escherichia coli (ETEC) was evaluated in mice primed with an intramuscularly delivered CFA/I-encoding DNA vaccine followed by two oral immunizations with a live recombinant Salmonella typhimurium vaccine strain expressing the ETEC antigen. The booster effect ...

Journal: :Journal of virology 2006
Natalia Fernandez-Borges Alejandro Brun J Lindsay Whitton Beatriz Parra Fayna Diaz-San Segundo Francisco J Salguero Juan M Torres Fernando Rodriguez

Transmissible spongiform encephalopathies (TSEs) can be ameliorated by prion protein (PrP)-specific antibodies, but active immunization is complicated by immune tolerance to the normal cellular host protein (PrP(C)). Here, we show that DNA immunization of wild-type mice can break immune tolerance against the prion protein, resulting in the induction of PrP-specific antibody and T-cell responses...

Journal: :The Journal of infectious diseases 1996
J J Donnelly D Martinez K U Jansen R W Ellis D L Montgomery M A Liu

Genital infections with human papillomavirus (HPV) are increasingly recognized as a significant source of human disease; HPV is now implicated in up to 90% of cervical carcinomas. Neutralizing antibodies against papillomaviruses recognize conformational epitopes formed when viral capsid proteins assemble into virions or virus-like particles. Immunization with plasmid DNA encoding the major vira...

Journal: :Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 1999
V Azevedo G Levitus A Miyoshi A L Cândido A M Goes S C Oliveira

DNA-based immunization has initiated a new era of vaccine research. One of the main goals of gene vaccine development is the control of the levels of expression in vivo for efficient immunization. Modifying the vector to modulate expression or immunogenicity is of critical importance for the improvement of DNA vaccines. The most frequently used vectors for genetic immunization are plasmids. In ...

2014
Rashmi Jalah Viraj Kulkarni Vainav Patel Margherita Rosati Candido Alicea Jenifer Bear Lei Yu Yongjun Guan Xiaoying Shen Georgia D. Tomaras Celia LaBranche David C. Montefiori Rajasekhar Prattipati Abraham Pinter Julian Bess Jeffrey D. Lifson Steven G. Reed Niranjan Y. Sardesai David J. Venzon Antonio Valentin George N. Pavlakis Barbara K. Felber

We tested the concept of combining DNA with protein to improve anti-HIV Env systemic and mucosal humoral immune responses. Rhesus macaques were vaccinated with DNA, DNA&protein co-immunization or DNA prime followed by protein boost, and the magnitude and mucosal dissemination of the antibody responses were monitored in both plasma and mucosal secretions. We achieved induction of robust humoral ...

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