Large-scale DNA sequencing projects can be limited by various technical bottlenecks. As more DNA templates are generated and successfully processed, the mechanisms and technologies continue to be improved and optimized. Fluorescence-based automated sequencing (1) has replaced radioisotope-based manual DNA sequencing (2) as the primary approach in large-scale genome projects. For this approach, ...

The chromatin structures of a variety of plasmids and plasmid constructions, transiently transfected into mouse Ltk- cells using the DEAE-dextran procedure, were studied by micrococcal nuclease digestion of nuclei and Southern hybridization. Although regularly arranged nucleosome-like particles clearly were formed on the transfected DNA, the nucleosome ladders, in some cases with 13-14 bands, w...

OBJECTIVES We performed a cytotoxicity study by cell magnetometry, measured lactate dehydrogenase (LDH) activity by enzyme assay, detected DNA ladder formation, and performed morphological examination by electron microscopy in order to evaluate the safety of high temperature wool (HT wool), an asbestos substitute, using long and short chrysotile fibers (CF) as positive controls and phosphate bu...

Apoptosis, or physiologic cell death, is an endogenous cellular process whereby senescent, DNA-damaged or diseased cells are eliminated from the body. Classically, apoptotic cells are identified by distinct morphological criteria in histological preparations (1). Apoptosis has been characterized biochemically by the activation of a nuclear endonuclease that cleaves the DNA into multimers of 180...

LIPN, UMR CNRS 7030. Institut Galilée Université Paris-Nord, 99, avenue Jean-Baptiste Clément 93430 Villetaneuse, France. 2 Université Pierre et Marie Curie, Laboratoire de Physique Théorique de la Matière Condensée, CNRS UMR 7600 Tour 16, 2 étage, 4, place Jussieu, F 75252 Paris Cedex 05, France. 3 H. Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences Division of Theoreti...

The synthesis and NMR structural studies are reported for a modular threading tetraintercalator bound to DNA. The tetraintercalator design is based on 1,4,5,8-tetracarboxylic naphthalene diimide units connected through flexible peptide linkers. Aided by an overall C(2) symmetry, NMR analysis verified a threading polyintercalation mode of binding, with linkers alternating in the order minor groo...

Miltefosine causes leishmanial death, but the possible mechanism(s) of action is not known. The mode of action of miltefosine was investigated in vitro in Leishmania donovani promastigotes as well as in extra- and intracellular amastigotes. Here, we demonstrate that miltefosine induces apoptosis-like death in L. donovani based on observed phenomena such as nuclear DNA condensation, DNA fragment...