e-MagnetoMethyl IP is a new method for electrochemical analysis of global DNA methylation. It avoids bisulfite treatment, PCR amplification, and enzyme-based signal generation can detect differences as low 5% in methylation levels.

background:estrogens play a substantial role in the proliferation, progression and treatment of breast cancer by binding with two estrogen receptors, alpha and beta (erα and erβ). resistance to endocrine therapy is a major problem in the treatment of breast cancers and, in some cases, may be related to loss of er gene expression. we have already showed that erα methylation occurs in high freque...

The human body consists of more than 200 different types cells, but all cells contain the same DNA code. How is it possible that code tells various to be different? information for life not only coded in DNA, also on DNA. Chemical attachments, which are called methylation, can “turn off” parts needed a specific cell. methylation influenced by our surroundings. Sometimes, changes might lead dise...

The variation of gene expression seeds or leaves Arabidopsis thaliana was investigated by irradiation with oxygen and air plasmas. plasma reported on the growth promotion induced consequence in plant cells neutral active species. increase leaf area ratio to due epigenetics such as activation DNA demethylation transcription factors enhancement effect inherited next-generation through cell divisi...

objective: to determine the role of global genome methylation in gastritis lesion and its relation with clinicopathologic finding. materials and methods: the study was conducted on 44 gastritis and normal adjacent specimens using a technique composed of restriction enzyme digestion and pyrosequencing known as luma (luminometric methylation assay). at first, dna extracted from gastritis lesion a...

Background & Objective: Papillary thyroid cancer (PTC) is considered to be the most common type of thyroid malignancies. Epigenetic alteration, in which the chromatin conformation and gene expression change without changing the sequence of DNA, can occur in some tumor suppressor genes and oncogenes. Methylation is the most common type of epigenetic alterations that can be an ex...

Objective: Methylation status of Septin9 (SEPT9) and vimentin (VIM) genes in circulating tumor DNA colorectal cancer (CRC) patients is a promising bio-marker for the early detection CRC. The aim present study was to identify methylation promoter regions SEPT9 VIM cohort Indian with biopsy proven cancer. Material Methods: Forty-five consecutive were recruited. 10 mL venous samples collected from...

Abstract In glioblastoma, treatment with radiation and chemotherapy leads to DNA-damage most DNA breaks are faithfully repaired, but the impact on epigenome is largely unknown. Using newly developed tools enable these studies, we hypothesize that genome-wide damage local alterations in DNA-methylation, genome organization, results persistent gene-expression near sites of repaired damage. We use...