More than 185 species, strains and unclassified Theileria parasites are categorized in the Entrez Taxonomy. The accurate diagnosis and proper identification of the causative agents are important for understanding the epidemiology, prevention and appropriate treatment. This study aims to discuss the importance of two genes of Theileria annulata 18S ribosomal RNA (18S rRNA) and Theileria annulata...

introduction: glycoprotein b (gb) is the major antigen for induction of humoral responses against human cytomegalovirus (hcmv) making it an attractive candidate for immune prophylaxis. in the present study, the humoral immune response of balb/c mice to a truncated hcmv gb protein fused with gfp was evaluated. methods: the truncated gb coding sequence was synthesized and cloned in pegfpn1 eukary...

objective(s):  denaturing high performance liquid chromatography (dhplc) is a high throughput approach for screening dna sequence variations. to assess oven calibration, cartridge performance, buffer composition and stability, the wave low and high range mutation standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. the purpose of this study was to provi...

aspartic proteases are a relatively small group of enzymes which express in various nematodes including onchocerca volvulus. an estimation of the gene copy number corresponding to the ov7a clone, which contains a cdna insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of o. volvulus, was made by slot blot analysis in a closely related species o. gibsonig...

Genetic Analysis of D-Loop Region Mitochondrial DNA Sequence in Iranian Patients with Familial Adenomatous Polyposis (FAP): A Case-Control Study

objective(s) in addition to several molecular methods and in particular 16s rdna analysis, the application of a more discriminatory genetic marker, i.e., 16s-23s internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. in the current study we aimed to apply this sequencing power to conclusive identification of some iranian clinical s...

background: thioredoxin reductase 1 (txnrd1) and thioredoxin interacting protein (txnip) also known as thioredoxin binding protein 2 or vitamin d3-upregulated protein 1 are key players in oxidative stress control. thioredoxin (trx) is one of the major components of the thiol reducing system and plays multiple roles in cellular processes. computational analyses of txnrd1, txnip and trx expressio...