An attempt was made to purify Clostridium perfringens Beta toxin. Crude toxin prepared by ammonium sulphate precipitation of culture supernatants was purified by chromatography on Sephadex G50, Sephadex G100 and DEAE cellulose. This material, although highly purified was not homogeneous on polyacrylamide gel electrophoresis. It had a toxicity of 800 000 mouse MLDs/mg N, a typical protein absorp...

The extracellular Opacity Factor elaborated by a strain of group A streptococcus M type 2 was purified by ammonium sulphate precipitation, DEAE-cellulose and hydroxylapatite column chromatography and Sephadex G-200 gel filtration. Gel filtration experiments indicated that the Opacity Factor is consituted of high molecular weight proteins or protein aggregates which appear to dissociate into sub...

background: detecting bence-jones protein in urine is essential for determining plasma cell dyscrasia and multiple myeloma. conventionally, acid-heat precipitation assay is used for detecting bence-jones protein in most medical laboratories; however, because of the low accuracy of this test, other more sensitive tests like urine electrophoresis are recommended.  materials and methods: in this s...

A hygromycin B phosphotransferase activity from Streptomyces hygroscopicus has been highly purified by ammonium sulphate fractionation followed by affinity column chromatography through Sepharose-6B-hygromycin-B. The combined active fractions showed a single protein band (41 kDa) when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. When gel electropho...

alpha 1-Proteinase inhibitor was purified from porcine blood by ammonium sulphate and Cibachron Blue-Sepharose fractionation, ion exchange chromatography on DEAE-Cellulose, gel filtration on Sephadex G-25, and zinc chelating chromatography. Thus, an inhibitor preparation with a specific activity of 1.62 IU/mg protein (enzyme: trypsin; substrate: BzArgNan) was obtained. In sodium dodecyl sulphat...

interaction between dna and histone h, was investigated in the presence and absence of sodium-n-dodecyl sulphate (sds) and dodecyl trimethylammonium bromide (dtab) at temperatures of 27 and 37?c, in 2.5 mm phosphate buffer, ph 6.4 by uv spectrophotometry, equilibriumdialysis and titration. the presence of 1.33 mm sds caused histone h to fold and to further contact dna. binding data were used in...

1. The alpha-fibrous protein (alpha-keratin) component of bovine epidermis has been extracted and characterized. 2. Prekeratin, a multichain unit of the epidermal tonofilaments, was shown to consist of six different polypeptide chains on polyacrylamide-gel systems containing sodium dodecyl sulphate or sodium decyl sulphate with discontinuous gel buffers, but only three chains were seen when a g...