To examine the functional role of CRH in the regulation of energy homeostasis by leptin, we measured the effects of the CRH antagonist, alpha-helical CRH 8-41 (alphaCRH) on a number of factors affected by leptin activity. These included food intake, body weight, hypothalamic c-fos-like immunoreactivity (c-FLI), weight and histological characterization of white adipose tissue, and mRNA expressio...

OBJECTIVES : The purpose of this study was to determine: 1. The spatial pattern of c-fos gene expression in rat hypothalamic neurons after exposure to NMS and 2. The expression of c-fos gene after combined exposure to NMS and EHF irradiation of the rat skin. METHODS : The experiments were performed on 28 male adult Sprague-Dawley rats. After rats were subjected to noxious mechanical stimulation...

Indirect immunohistochemistry and an androgen receptor antibody. PG-21, was used to characterize rhe onlogeny of cells containing androgen receptor-like immunoreactivity (AR-ir) and the effects of exogenous testosterone administration on AR-ir in the developing Brazilian opossum brain. Cells containing AR-ir were first seen in the anterior pituitary at day 5 of postnatal life (PN). Between 10 a...

Fibers projecting from several levels of the spinal cord to the diencephalon and telencephalon were labeled anterogradely with Phaseolus vulgaris leucoagglutinin injected iontophoretically. Labeled fibers in the thalamus confirmed projections previously observed. In addition, many labeled fibers were seen in the hypothalamus and in telencephalic areas not generally recognized previously as rece...

In nature and under laboratory conditions, dams nurse rabbit pups once daily for a duration of fewer than 5 min. The present study explored neural mechanisms mediating the timing of nursing in this natural model of food anticipatory activity, focussing on the suprachiasmatic nucleus (SCN), the locus of the master circadian clock and on the dorsomedial hypothalamic nucleus (DMH), a region implic...

The modification in the activity of noradrenergic neurons projecting to the hypothalamus and the pituitary-adrenal response during morphine withdrawal as well its correlation with alterations in corticotropin-releasing factor (CRF) and vasopressin (AVP) content in different brain areas was analyzed. Male rats were implanted with placebo (naïve) or morphine (tolerant/dependent) pellets for 7 day...

The dorsal premammillary nucleus (PMd) is thought to play a critical role for the expression of fear responses to environmental threats. We have reported previously that during an encounter with a predator the PMd presents an impressive increase in Fos levels and cell body-specific chemical lesions therein virtually eliminate the expression of escape and freezing responses. In the present study...

The effects of running wheel exercise and caloric restriction on the regulation of body weight, adiposity, and hypothalamic neuropeptide expression were compared in diet-induced obese male rats over 6 wk. Compared with sedentary controls, exercising rats had reduced body weight gain (24%), visceral (4 fat pads; 36%) and carcass (leptin; 35%) adiposity but not insulin levels. Hypothalamic arcuat...