We investigated the natural variation in silk composition and mechanical performance of the orb-weaving spider Argiope trifasciata at multiple spatial and temporal scales in order to assess how protein composition contributes to the remarkable material properties of spider dragline silk. Major ampullate silk in orb-weaving spiders consists predominantly of two proteins (MaSp1 and MaSp2) with di...

In the surface mining of mineral deposits, land resources suitable for agricultural purposes are inappropriately spent in large volumes. When deep open pits, overburden rocks mainly transported to surface. The optimal solution reducing area disturbed lands is placement internal dumps pit. This especially when a deposit with several pits where at least one them depleted. Therefore, it important ...

Albeit silks are fairly well understood on a molecular level, their hierarchical organisation and the full complexity of constituents in the spun fibre remain poorly defined. Here we link morphological defined structural elements in dragline silk of Nephila clavipes to their biochemical composition and physicochemical properties. Five layers of different make-ups could be distinguished. Of thes...

Background Spider dragline silk is considered to be the toughest biopolymer on Earth due to an extraordinary combination of strength and elasticity. With synthetic biology it is possible to express recombinant spider silk proteins, which are characterized by a highly repetitive rich glycine and alanine sequence [1]. However, production of high molecular weight spider silk protein can be difficu...

Abstract Bacterial infections are well recognised to be one of the most important current public health problems. Inhibiting adhesion microbes on biomaterials is approach for preventing inflammation. Coatings made recombinant spider silk proteins based consensus sequence Araneus diadematus dragline fibroin 4 have previously shown microbe-repellent properties. Concerning silicone implants, it ha...