BACKGROUND Production of native antigens for serodiagnosis of helminthic infections is laborious and hampered by batch-to-batch variation. For serodiagnosis of echinococcosis, especially cystic disease, most screening tests rely on crude or purified Echinococcus granulosus hydatid cyst fluid. To resolve limitations associated with native antigens in serological tests, the use of standardized an...

Correspondence: Dr. Ömer Etlik Yuzuncu Yil Üniversitesi, Tıp Fakultesi Hastanesi, Radyoloji Anabilim Dalı 65200 Van, Turkey Phone: +904322143752 Fax: +904322167519 E-mail: [email protected] INTRODUCTION Echinococcosis is an infectious disease caused by the larval form of the genus Echinococcus. Two of the parasite species may cause severe disease in humans: E. granulosus and E. alveolaris (...

BACKGROUND Cystic echinococcosis (CE) is a globally distributed cestode zoonosis that causes hepatic cysts. Although Echinococcus granulosus sensu stricto (s.s.) is the major causative agent of CE worldwide, recent molecular epidemiological studies have revealed that E. canadensis is common in countries where camels are present. One such country is Mongolia. METHODOLOGY/PRINCIPAL FINDINGS For...

BACKGROUND Echinococcosis in humans is a disease caused by the larvae of Echinococcus granulosus (E. granulosus) and Echinococcus multilocularis (E. multilocularis). Serological tests are valuable, especially in the clarification of unexplained clinical findings and imaging methods. For this reason, indirect hemagglutination (IHA), latex agglutination, immunoelectrophoresis, immunoblotting, imm...

cystic echinococcosis is an important zoonosis in the sheep rising areas of iran. to develop a simple and reliable diagnostic method for echinococcus infection in definitive hosts, e. granulosus polyoclonal antibodies (polyabs) were prepared from adult worm in rabbit. a selected poab was used for coproantigen detection in faecal samples obtained from animals naturally infected with echinococcus...

BACKGROUND Echinococcus granulosus is a cestode whose larval stage causes cystic echinococcosis in wild animals, livestock, and human. OBJECTIVE The aim of this study is to highlight the seroepidemiology of E. granulosus infection and surgical cases in the general population of Khorram Abad district, southwest of Iran. MATERIALS AND METHODS Anti-E. granulosus antibodies were tested in 617 i...

In this study, molecular characterization of Echinococcus granulosus sample obtained from water buffalo originating from southwest of Iran was performed using comparative sequence analysis of cox1 mitochondrial gene. DNA was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in Khuzestan province. Molecular and phylog...

Alveolar and cystic hydatidosis are caused by infection with the larval stages of Echinococcus multilocularis and Echinococcus granulosus, respectively. A host-protective antigen has been identified in E. granulosus. Here we identify the presence of a closely related protein in E. multilocularis, characterize and express a cDNA encoding the antigen (designated EM95), determine the structure of ...

background: despite echinococcus granulosus , there are merely two old reports of e. multilocularis in-fection among iranian canids of moghan plain, the only area known endemic for the species. we detected specific dna markers in fecal samples by pcr (copro-pcr) for differential diagnosis of echinococcus species in living canids. methods: totally 144 fecal samples from domestic dogs, red foxes ...

The cases of nine Polish patients with diagnosed cystic echinococcosis (CE) were examined. A total of nine isolates obtained postoperatively were investigated using PCR and sequencing. The mitochondrial region of nad1 gene was amplified. This PCR and sequencing analysis revealed the presence of Echinococcus canadensis G7 in seven patients and E. granulosus G1 in two patients. These data demonst...