High fidelity replicative DNA polymerases are unable to synthesize past DNA adducts that result from diverse chemicals, reactive oxygen species or UV light. To bypass these replication blocks, cells utilize specialized translesion DNA polymerases that are intrinsically error prone and associated with mutagenesis, drug resistance, and cancer. How untimely access of translesion polymerases to DNA...

The dinB gene of Escherichia coli is known to be involved in the untargeted mutagenesis of lambda phage. Recently, we have demonstrated that this damage-inducible and SOS-controlled gene encodes a novel DNA polymerase, DNA Pol IV, which is able to dramatically increase the untargeted mutagenesis of F' plasmid. At the amino acid level, DNA Pol IV shares sequence homologies with E. coli UmuC (DNA...

Escherichia coli pol V (UmuD'(2)C), the main translesion DNA polymerase, ensures continued nascent strand extension when the cellular replicase is blocked by unrepaired DNA lesions. Pol V is characterized by low sugar selectivity, which can be further reduced by a Y11A "steric-gate" substitution in UmuC that enables pol V to preferentially incorporate rNTPs over dNTPs in vitro. Despite efficien...

In this paper, a novel gold film-coated V-shape dual-core photonic crystal fiber (V-DC-PCF) polarization beam splitter (PBS) based on surface plasmon resonance effect is proposed. The coupling lengths of the X-polarization (X-pol) and Y-polarization (Y-pol) corresponding length ratio proposed V-DC-PCF PBS without film with are compared. structure parameters thickness optimized through investiga...

This paper proposes a novel liquid crystal-filled, dual core photonic crystal fiber polarization beam splitter (LC-DC-PCF PBS) based on the coupled mode theory of DC-PCF. The birefringence odd and even modes, coupling lengths (CLs) X-polarization (X-pol) Y-polarization (Y-pol), corresponding length ratio (CLR) proposed LC-DC-PCF PBS filled without LC E7 with are compared. change rules CLs X-pol...

Polarimetric SAR interferometry (Pol-InSAR) is a radar remote sensing technique that is sensitive to the vertical distribution of scattering processes in volumes. The Random Volume over Ground (RVoG) model is a powerful tool used to invert forest height from PolInSAR data. But Pol-InSAR inversion performance depends critically on uncompensated decorrelation contributions (i.e. temporal decorrel...

DNA polymerase (Pol ) is the only one of the fifteen human DNA-dependent DNA polymerases for which a natural occurring deficiency is known to predispose humans to cancer. Xeroderma pigmentosum (XP) is an autosomal recessive genetic disorder, associated with a greatly increased risk of sunlightinduced skin tumors, and individuals with the variant type of this syndrome, XP-V, have defects in Pol ...

When the replication fork moves through the template DNA containing lesions, daughter-strand gaps are formed opposite lesion sites. These gaps are subsequently filled-in either by translesion synthesis (TLS) or by homologous recombination. RecA filaments formed within these gaps are key intermediates for both of the gap-filling pathways. For instance, Pol V, the major lesion bypass polymerase i...

INTRODUCTION Morphea or localized scleroderma is a relatively rare disease whose main symptom is excessive skin fibrosis. Here we focus on the involvement of human endogenous retroviruses (HERVs) in morphea. The HERVs are a vast and intensely growing field in genomics. HERVs are of special interest as far as autoimmune disorders are concerned, yet little effort has been made until now to assess...

The aim of this work was the preparation and characterization of scaffolds with mechanical and functional properties able to regenerate bone. Porous scaffolds made of chitosan/gelatin (POL) blends containing different amounts of a bioactive glass (CEL2), as inorganic material stimulating biomineralization, were fabricated by freeze-drying. Foams with different compositions (CEL2/POL 0/100; 40/6...