An enzyme-linked immunosorbent assay (ELISA) and the hemagglutination inhibition (HI) test were used to evaluate the response of laboratory rats to experimental infection with pneumonia virus of mice. The ELISA procedure was more sensitive than the HI test and detected very low levels of antibodies early in the course of infection. At 5 days postinfection, ELISA detected antibody increases in f...

BACKGROUND Brucellosis is a world-wide disease, which has a diverse clinical manifestation, and its diagnosis has to be proven by laboratory data. Serum agglutination test (SAT) is the most-widely used test for diagnosing brucellosis. The enzyme linked immunosorbent assay (ELISA) can also determine specific antibody classes against brucella. It is a sensitive, simple and rapid test, which could...

A total of 255 sera were collected from unvaccinated crossbred (174) and indigenous (81) cattle and buffalo having history of respiratory and reproductive disorders. All the sera sample were subjected to indirect ELISA and passive haemagglutination (PHA) test for the detection of BHV-1 antibodies. Indirect ELISA and PHA test detected significantly (P<0.01) varied prevalence of BHV-1 antibody i....

An evaluation of a rapid test for the presence of Helicobacter pylori from stool specimens looking for antigen proceeded for a period of 8 months from March to November 2011. A total of 212 stool samples were collected from 2 different hospitals in Holy Makkah City. Twenty six patients were positive for H. pylori by the ELISA test which was used as the gold standard. The rapid test when compare...

BACKGROUND Leptospirosis is diagnosed on clinical grounds, and confirmed by microscopic agglutination test (MAT). IgM-ELISA (Serion-Virion) and immunochromatography test (Leptocheck-WB) are two immunodiagnostic assays for leptospirosis. Their sensitivity, specificity and applicability in Sri Lanka have not been systematically evaluated. METHODS Clinically diagnosed leptospirosis patients (n =...

A rapid ELISA test for detection, characterization and quantification of human paraproteins was developed. The proposed method is a sandwich ELISA, where the capture antibody is specific for a given heavy chain (gamma, alpha or mu) and the labelled antibody is specific either for kappa or for lambda light chain. Both standard and patient sera are tested with all six possible antibody combinatio...

APPLICATION OF ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN RIO GRANDE DO SUL, BRAZIL. The results of an indirect ELISA (I-ELISA) and a competitive ELISA (C-ELISA) for detection of antibody to Brucella abortus in cattle were compared with those of conventional serological tests. The sensitivity of I-ELISA using 230 sera from infected animals was 98.6% and the sp...

The aim of the present study was to evaluate the serological methods using ELISA with recombinant-rK39 (ELISA-rK-39) and soluble extract-SE (ELISA-SE) antigens, the indirect fluorescence antibody test (IFAT) in comparison to an immunochromatography rapid diagnostic test (RDT-rK39) and with a direct parasitological exam (PA) for Canine Visceral Leishmaniasis (CVL) diagnosis. The results showed t...