organogenesis and neoformant plantlet production were studied by culturing the different parts of mature embryo including radicle and proximal and distal parts of cotyledon of mission and kroneiki cultivars. different parts of embryos were cultures in omc medium containing growth regulators, such as indole butyric acid (iba) and 2-isopentenyl adenine (2-ip) or iba and naphthalene acetic acid (n...

Primary cassava somatic embryos were induced on a medium without CaCl(2), however, no or only a few secondary somatic embryos were formed from them. With 15 mM CaCl(2) in the medium for induction of cassava primary embryos, more secondary somatic embryos were produced from them, and they were much effective in maintaining their embryogenic capacity than the controls of embryos which were induce...

V Girijashankar1*, KK Sharma2, P Balakrishna and N Seetharama 1. Centre for Biotechnology, Jawaharlal Nehru Technological University (JNTU), Kukatpally, Hyderabad 500 072, Andhra Pradesh, India 2. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India 3. National Research Centre for Sorghum (NRCS), Rajendranagar, Hyderabad 500 030, ...

Somatic embryogenesis is an asexual form of plant propagation in nature that mimics many of the events of sexual reproduction. Also, this process may be reproduced artificially by the manipulation of tissues and cells in vitro. Some of the most important factors for a successful plant regeneration are the culture medium and environmental incubation conditions. In vitro somatic embryogenesis is ...

Clonal production of loblolly pine (Pinus taeda L.) through somatic embryogenesis has great potential to meet the increasing industrial demands for highquality uniform raw materials. A major barrier to the commercialization of this technology is the low quality of resulting embryos. Twenty-five newly initiated loblolly pine genotypes were followed through the process of liquid culture establish...

Somatic embryogenesis is an important biotechnological tool that demonstrates significant benefits when applied to forest tree species; clonal propagation, cryostorage of valuable germoplasm and genetic transformation are among the most promising of its applications. In this chapter, the state of the art of somatic embryogenesis in chestnut (an important economical tree species of the genus Cas...