The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic em...

Wound healing is a series of complex events to heal and regenerate epidermal and dermal tissues after injury. Adults often heal their wounds with scars, while embryos can accomplish this process perfectly, without scars. Previous studies have provided some comparisons between adult and embryonic wound healings; however, details in the inflammatory phase of wound healing, which is believed to be...

Wound healing is a series of complex events to heal and regenerate epidermal and dermal tissues after injury. Adults often heal their wounds with scars, while embryos can accomplish this process perfectly, without scars. Previous studies have provided some comparisons between adult and embryonic wound healings; however, details in the inflammatory phase of wound healing, which is believed to be...

To address the regenerative capability of the differentiating hindbrain, we ablated the cerebellum in wild-type and transgenic zebrafish embryos. These larvae showed no obvious locomotive malfunction several days after the ablation. Expression analysis and in vivo time-lapse recording in GFP (green fluorescent protein)-transgenic embryos indicate that cerebellar neuronal cells can regenerate fr...

Upon entry into mitosis, many microtubules are nucleated that coordinately integrate into a stable, yet dynamic, mitotic spindle apparatus. In a recent publication, we examined microtubule-generating pathways within a single model system, the Drosophila syncytial embryo. We found that, following depolymerisation of metaphase spindle microtubules by cold treatment, spindles regenerate predominan...

Wound healing is a series of complex events to heal and regenerate epidermal and dermal tissues after injury. Adults often heal their wounds with scars, while embryos can accomplish this process perfectly, without scars. Previous studies have provided some comparisons between adult and embryonic wound healings; however, details in the inflammatory phase of wound healing, which is believed to be...

Background: Studies of preimplantation stage embryos by classic cytogenetic techniques have limitations, starting with the need for good metaphase stage when only one third of all analyzed embryos may show good quality metaphases. The incidence of chromosome anomalies in embryos produced by in vitro procedures is generally higher than that of embryos formed in vivo. Pressure specifically affect...

Regeneration of the zebrafish caudal fin following amputation occurs through wound healing, followed by formation of a blastema, which produces cells to replace the lost tissue in the final phase of regenerative outgrowth. We show that ptpn11a-/- ptpn11b-/- zebrafish embryos, lacking functional Shp2, fail to regenerate their caudal fin folds. Rescue experiments indicated that Shp2a has a functi...

In general, human tissues have a very limited potential to regenerate. However, recent progress in stem cell research and in tissue engineering promises novel prospects for tissue regeneration in dental practice in the future. Stem cells have been discovered in many adult tissues, including teeth, and stem cells from embryos have the potential to form all adult tissues. Embryonic stem cells can...