Lymphocyte migration from the blood to sites of tissue injury is mediated, in part, through the interaction of these cells with endothelial cells lining the vessel walls. The ability of endothelial cells to produce nitric oxide may be important in this process. We found that the addition of the nonspecific lymphocyte activators lipopolysaccharide (LPS) or concanavalin A (Con A) to rat hepatic e...

Goodwin BL, Pendleton LC, Levy MM, Solomonson LP, Eichler DC. Tumor necrosis factorreduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells. Am J Physiol Heart Circ Physiol 293: H1115–H1121, 2007. First published May 11, 2007; doi:10.1152/ajpheart.01100.2006.—Endothelial dysfunction associated with elevated serum levels of TNFobserved in diabetes, ob...

We previously reported that angiotensin II stimulates an increase in nitric oxide production in pulmonary artery endothelial cells. The aims of this study were to determine which receptor subtype mediates the angiotensin II-dependent increase in nitric oxide production and to investigate the roles of the angiotensin type 1 and type 2 receptors in modulating angiotensin II-dependent vasoconstric...

In this issue of the Journal, Gallo et al. (1) report an interesting analysis of nitric oxide production and angiogenesis in head and neck cancers. They observed the following: 1) Tumor levels of total nitric oxide synthase activity (including inducible nitric oxide synthase activity) and of 38,58 cyclic guanosine monophosphate (cGMP) (a known mediator of cellular response to nitric oxide) were...

RATIONALE The neuropeptide secretoneurin induces angiogenesis and postnatal vasculogenesis and is upregulated by hypoxia in skeletal muscle cells. OBJECTIVE We sought to investigate the effects of secretoneurin on therapeutic angiogenesis. METHODS AND RESULTS We generated a secretoneurin gene therapy vector. In the mouse hindlimb ischemia model secretoneurin gene therapy by intramuscular pl...

The association between Escherichia coli endotoxin-induced organ damage and nitric oxide-related mechanisms was investigated in the spleen of male Swiss albino mice (20-40 g) by using (1) Pt/Ir electrochemical sensor connected to an amperometric detection system (NO-501, InterMedical Co., Japan), (2) nitrotyrosine immunohistochemistry, (3) conventional light microscopy and (4) immunoblotting te...

1. Rossi GP, Cesari M, Zanchetta M, et al. The TC endothelial nitric oxide synthase genotype is a novel risk factor for coronary artery disease in Caucasian patients of the GENICA study. J Am Coll Cardiol 2003;41:930–7. 2. Alvarez R, Gonzalez P, Batalla A, et al. Association between the NOS3 (-786 T/C) and the ACE (I/D) DNA genotypes and early coronary artery disease. Nitric Oxide 2001;5:343–8....