Enzymes are remarkable catalysts that lie at the heart of biology, accelerating chemical reactions to an astounding extent with extraordinary specificity. Enormous progress in understanding the chemical basis of enzymatic transformations and the basic mechanisms underlying rate enhancements over the past decades is apparent. Nevertheless, it has been difficult to achieve a quantitative understa...

BACKGROUND Phospholipase A1 is an enzyme that hydrolyzes phospholipids at the sn-1 position. It has potential applications across diverse fields including food, pharmaceutical, and biofuel industries. Although there has been increasing interest in the use of phospholipase A1 for degumming of plant oils during biodiesel production, production of recombinant phospholipase A1 has been hampered by ...

Wild Silkmoth cocoons are difficult or impossible to reel under conditions that work well for cocoons of the Mulberry silkmoth, Bombyx mori . Here we report evidence that this is caused by mineral reinforcement of Wild Silkmoth cocoons and that washing these minerals out allows for the reeling of commercial lengths of good quality fibers with implications for the development of the "Wild Silk" ...

Life as we know it heavily relies on biological catalysis, in fact, in a very nonromantic version of it, life could be considered as a series of chemical reactions, regulated by the guarding principles of thermodynamics. In ancient times, a beating heart was a good sign of vitality, however, to me, it is actually the presence of active enzymes that counts… Though we do not usually pay attention...

Silk fibroin is a protein with unique combination of properties and widely studied for biomedical applications. The extraction (degumming) from the silk filament impacts outcoming material. degumming can be conducted different procedures. Among them, most used procedure in research field alkali sodium carbonate (Na2CO3). In this study, by use statistical method, namely, design experiment (DOE),...

<p>Kesambi seed oil (<em>Schleichera oleosa</em>) is one of the resources that can be utilized as an alternative to substitute palm in cooking production due its lower moisture content, peroxide value, and saponification number. However, high level free fatty acid (FFA) cyanide (HCN) contents kesambi requires further purification prior commercialization. In this study, process...

Bast fiber plants require a post-harvest process to yield useable natural cellulosic fibers, denoted as retting or degumming. It encompasses the degradation of cell wall’s non-cellulosic gummy substances (NCGs), facilitating fibers separations, setting fiber’s quality, and determining downstream usages. Due inconvenience traditional practices, bacterial inoculum enzyme applications for gained a...

Removing sericin from the periphery of silk without damage to fibroin (SF) obtain high-molecular-weight SF is a major challenge in field SF-based biomaterials. In this study, four neutral proteases, subtilisin, trypsin, bromelain and papain, were used degum silk, degumming efficiency proteases their influence on molecular weight (MW) regenerated studied. The results indicated that all could rem...