An enzymatic method has been developed to simultaneously determine glucose, maltose and sucrose in tubers The principle of the method is based on an enzymatic cleavage of these disaccharides and specific measurement the resulting glucose using an enzymatic procedure described by Holm (3). The study comprises two parts; the first one includes modifications of the pretreatment method to minimize ...

In this improved method for rapid enzymatic determination of total cholesterol, the lipid extracts from tissues are dissolved in an equal volume of peroxide-free dioxane/isopropanol (50/50 by vol) solution, and are reacted directly with the aqueous enzymatic reagent, without further treatment. The presence of organic solvents, such as dioxane and isopropanol, does not interfere with enzymatic a...

Methods 30 patients with severe sepsis or septic shock were studied at ICU admission and after 24 and 48 hours. Immune cells were identified and isolated using an immunomagnetic positive cell isolation procedure (Dynabeads for Human Monocytes, Human B cells, Human CD4 cells, Invitrogen Dynal AS, Oslo, Norway). Enzymatic activity of mitochondrial complexes I, IV, and ATP synthase and ATP content...

Featured Article: Carr TP, Andresen CJ, Rudel LL. Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts. Clin Biochem 1993;26:39 – 42. The need to measure serum or plasma cholesterol prompted the development of enzyme-based colorimetric assays during the 1970s. These assays eventually replaced the traditional chemical methods. The advantages o...

Most surgeons seem to favour the former method. Even when the erisophake is used instead of forceps, the technique of zonulolotomy and delivery of the lens remains essentially the same. Since the introduction of A.C.T., however, mechanical zonulolotomy has been replaced by enzymatic zonulolysis and the extraction of the lens by seizing it at the 12 o'clock position is being increasingly practis...

BACKGROUND Conventional reprocessing of endoscopes with high-level disinfection is labor intensive, expensive, delays the turnover of instruments, and involves potential exposure of personnel to toxic chemicals. We report a prospective clinical trial with rigorous microbiologic assessment of a novel disposable, sterile, polyurethane sheath, which can be easily and snugly applied over a nasophar...

HE ESTABLISHMENT AND ACCEPTANCE of an analytic procedure as a method to be relied upon with complete confidence demands: (1) 8pecific’tty of the method, i.e. minimal interference by other compounds; (2) quantitative recovery of the specific substance when it is added to the medium in which it is usually determined; and (3) reproducibility of results within reasonable limits on replicate samples...

a long-term study was conducted between 1985 and 2003 on rehabilitation trials at sishen ironore mine, south africa, to identify grass species that would survive in the artificial growth medium appliedto the sites, and to determine the most suitable medium for sustainable vegetation growth. vegetationestablishment was tested at slopes of 18° and 34° and with five different cover materials. afte...