The enzymatic transesterification of ethyl ferulate (EF) with fish oil from cod liver was investigated with Novozym 435 as catalyst under solvent-free conditions. The purpose of the study is to evaluate the synthesis system for the production of feruloyl fish oil in industry. The modified HPLC method was first set up to characterise the reaction products together with liquid chromatography elec...

Enzymatic transesterification of soybean oil with methanol and ethanol was studied. Of the nine lipases that were tested in the initial screening, lipase PS from Pseudomonas cepacia resulted in the highest yield of alkyl esters. Lipase from Pseudomonas cepacia was further investigated in immobilized form within a chemically inert, hydrophobic sol-gel support. The gel-entrapped lipase was prepar...

BACKGROUND The biodiesel production can be carried out by transesterification using either chemical or enzymatic process. The enzymatic transesterification is more promising as it offers an environmental friendly option compared to the chemical process, where the lipases with high catalytic efficiency and good stability play a key role. Hence, it is of great value to identify novel lipases whic...

Introduction The fatty acid esters synthesis by transesterification of oils to produce biodiesel commonly involves methanol or ethanol as acyl acceptor. The transesterification of vegetable oils catalyzed by lipases is an alternative process for obtaining biodiesel. These biocatalysts working under mild conditions of temperature, allow for easy recovery of glycerol, synthesis of specific alkyl ...

BACKGROUND Ultrasound has been used to accelerate the rates of numerous chemical reactions, however its effects on enzymatic reactions have been less extensively studied. While known to result in the acceleration of enzyme-catalysed reactions, ultrasonication has also been shown to induce enzyme inactivation. In this study we investigated the effects of ultrasonic pretreatment on lipases in bot...

Enzymatic transesterification of soybean oil with methanol and ethanol was studied. 0f the nine lipases that were tested in the initial screening. lipase PS from Pseudornonas cepacia resulted in the highest yield of alkyl esters. Lipase from Pseudornonas cepacia was further investigated in immobilized from with in chemically inert, hydrophobic sol-gel support. The gel-entrapped lipase was prepa...

BACKGROUND Food industries aim to replace trans fat in their products by formulations having equivalent functionality and economic viability. Enzymatic transesterification can be a technological option to produce trans free fats targeting commercial applications. RESULTS Palm stearin and palm olein blends in different ratios were enzymatically transesterified in a solvent free system using a ...

Between 1930 and 1975 biochemical and structural analysis of enzymes led to a clear set of ideas that might form a basis for detailed understanding of enzyme action. Further development required energetic and thermodynamic analysis of enzymes in an aqueous medium, beyond the computational power then available. Structural enzymology advanced in other directions, but the fundamental questions of ...