Background: Collagen, the most abundant protein in the human body, and as an extracellular matrix protein, has an important role in the fiber formation. This feature of the collagen renders  establishment of the structural skeleton in tissues. Regarding specific features associated with the collagen, such as, formation of the porous structure, permeability and hydrophilicity, it can also be use...

Alpha amylases have various applications in different industries. Immobilization of alpha -amylase produced by a strain of Bacillus sp isolated from soil of Dibrugarh District of Assam, India was studied. Partially purified enzyme with 4062.62U/ml activity was used for immobilization study. 4% Sodium alginate was used for immobilization of enzyme and the optimum temperature, pH and incubation p...

Immobilization technology creates exciting new opportunities for commercial development in a wide range of industries. There are several reasons for using an enzyme in an immobilized form. In addition to more convenient handling of the enzyme, it provides for its facile separation from the product, thereby minimizing or eliminating protein contamination of the product. To date, pectinases have ...

Immobilization of α-Amylase produced by a Pseudomonas sp isolated from Indian Chicken feather was studied. Partially purified enzyme with 486.77 IU and specific activity of 811.28 units mg (protein) was used for immobilization study. An easily available and inexpensive 3% Sodium alginate matrix with easy immobilization gel entrapment procedure was used for trapping the enzyme showed 555.5 IU ac...

The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functi...

Glycoside hydrolases (GH) are enzymes capable to hydrolyze the glycosidic bond between two carbohydrates or even between a carbohydrate and a non-carbohydrate moiety. Because of the increasing interest for industrial applications of these enzymes, the immobilization of GH has become an important development in order to improve its activity, stability, as well as the possibility of its reuse in ...

A comparison between several acetylcholinesterase (AChE) immobilization procedures on the 7,7,8,8-tetracyanoquinodimethane (TCNQ)modified graphite working electrodes is presented. The immobilization methods employed crosslinking with glutaraldehyde in presence of BSA protein and photopolymerization with poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ). The main variations were rela...

Polyamide matrices, such as membranes, gels and non-wovens, have been applied as supports for enzyme immobilization, although in literature he enzyme immobilization on woven nylon matrices is rarely reported. In this work, a protocol for a Trametes hirsuta laccase immobilization sing woven polyamide 6,6 (nylon) was developed. A 24 full factorial design was used to study the influence of pH, spa...

During recent decades, the use of enzymes or chemoenzymatic cascades for organic chemistry has gained much importance in fundamental and industrial research. Moreover, several enzymatic reactions have also served green sustainable manufacturing processes especially fine chemicals, pharmaceutical, flavor/fragrance industries. Unfortunately, only a few been applied at scale because low stabilitie...

Encapsulation and immobilization technology is important for the food processing and bioengineering industries. Chitosan is a natural polysaccharide prepared by the N-deacetylation of chitin. It has been widely used in food and bioengineering industries, including the encapsulation of active food ingredients, in enzyme immobilization, and as a carrier for controlled drug delivery, due to its si...