Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA) are the two most useful and sensitive methods to measure the ng/ml to pg/ml ordered materials in the solution, such as in tissue, serum, urine and cultured cells, etc, and they are especially widely used in protein detection. This article describes the principle theories and techniques for WB and ELISA procedure. [Ma H, Yang Y....

This study aims to determine of Chloramphenicol test for Vannamei shrimp conducted ELISA (Enzyme-Linked Immunosorbent Assay) method with 1 treatment. is done by adding the Abenzyme conjugate, and ends addition substrate reaction stop buffer. Each treatment was performed three times. The value (CAP) (Litopenaeus vannamei) which tested at 4 weeks ranged from 0.021 0.153 ppb. result showed in stil...

background while hepatitis e virus (hev) mostly causes self-limited disease in general population, it is more severe in pregnant women. objectives this study aimed to investigate the seroprevalence of anti-hev igg among a population of pregnant women in west azerbaijan of . patients and methods one hundred thirty six pregnant women referred to urban health centers of urmia for pursuing pregnanc...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

results: of the 50 samples tested by the elisa method, 26 samples (52%) were positive for igg . no samples were positive for igm. of the 50 samples tested by the chemiluminescence method, 28 samples (56%) were positive for igg. no samples were positive for igm. conclusions: a significant relationship between the age of the youngest child and the infection rate was seen. no significant correlati...

Antigen tests for infectious diseases are inexpensive and easy-to-use, but the limit of detection (LOD) is generally higher than that PCR tests, which considered gold standard. In present study, we combined a sandwich enzyme-linked immunosorbent assay (ELISA) with thionicotinamide-adenine dinucleotide (thio-NAD) cycling to improve LOD antigen coronavirus disease 2019 (COVID-19). For recombinant...