Objective(s): Although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed.   Materials and Methods: Single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. The isolate showed much ...

background and objectives: lipases are particularly important due to the fact that they specifically hydrolyze acyl glycerol, oils and greases, which is of great interest for different industrial applications. materialst and methods : in this study, several lipase-producing bacteria were isolated from wastewater of an oil processing plant. the strain possessing the highest lipase activity was i...

sugar beet pulp is one of the sugar industries by-products which can be used for cellulase enzyme production, enzymatic saccharification, and alcohol production due to its high percentage of lingo-cellulosic content. trichoderma spp. is an important fungus that produces a wide range of cellulytic enzymes. in this study, cellulase enzyme was produced by placing sugar beet pulp in trichoderma fer...

This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isoprop...

Pseudomonas aeruginosa BBRC-10036 was used for lipase production. The organism secreted the enzyme extracellulary. In order to purify the enzyme, precipitation was done first, and then this lipase has been purified by Ion exchange Chromatography leading to 2.3-fold purification and 11.47% recovery. Lipase from P.aeruginosa was entrapped into Ca-alginate gel beads and effect of independent varia...

Bacillus sp. GUF8, isolated from acidic soil samples of a tea farm was identified as Bacillus cereus, based on 16S rDNA sequencing and standard bacterial identification methods. Following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. Consequently, thermostability and kinetic parameters of the purified enzyme wer...

objective(s): although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed.   materials and methods: single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. the isolate showed much greater endo-1, 4...

In this study, Glucoamylase production by Aspergillus Niger was investigated under solid state conditions with low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Different additions of (CF) were tested and WB+ 10% CF showe...