The aim of this study is to evaluate the effect biochar on tomato production under controlled conditions. Trials were installed using a complete randomized block design (CRD) with 5 replications 4 treatments: T0 (0%); T1 (8%); T2 (12%) and T3 (16%). During plant growth, parameters including height, diameter, number leaves, fruits biomass collected. Plants obtained from pot that received (T1, T3...

Commercial fungal amylase (diastase) has been immobilized through diazotization onto zirconia coated arylamine glass beads, affixed inside a plastic beaker. The immobilized enzyme retained 73.3% of the initial activity of free enzyme with a conjugation yield of 8 mg/g. Optimum pH of enzyme was decreased, while temperatures for maximum activity, energy of activation, time of incubation and Km fo...

We compared two methods for the direct selective measurement of hepatic lipase and lipoprotein lipase activities in human plasma after intravenous administration of heparin. Except for the emulsifier (gum arabic vs lecithin), the two assay media for hepatic lipase are essentially similar. Results for hepatic lipase by these two assays correlate well (r = 0.99). The assays for lipoprotein lipase...

The rate and extent of return of enzymic activity during oxidation of the sulfhydryl groups of reduced ribonuclease were studied under a variety of conditions. The rate of reactivation was found to be inversely proportional to the concentration of reduced protein. Under the most favorable conditions employed in this study (0.01 mg. protein/ ml., pH 8.2, 24” C.), enzymic activity was demonstrabl...

The Clitoria ternatea, Solanum nigrum and Aloe vera leaves were extracted serially by the solvents of increasing polarity (petroleum ether, chloroform and ethanol) were tested for their free radical scavenging activity against DPPH. The petroleum ether extracts of Clitoria ternatea, Solanum nigrum and Aloe vera leaves were the most effective scavenging of DPPH. These plant extracts were also an...

The role of Pseudomonas aeruginosa exotoxin A (ETA) as a virulence factor in the lung infections of cystic fibrosis (CF) patients is not well understood. Transcript-accumulation studies of bacterial populations in sputum reveal high levels of transcription of toxA, which encodes ETA, in some patients with CF. However, in general, tissue damage in the lungs of patients with CF does not seem to b...

These investigations were undertaken to discover whether the membrane of the mature human erythrocyte is capable of metabolic activity that could possibly provide the means for active transport. Hoffman (2) has clearly shown that ATP provides energy for sodium transport, and it has been assumed that ATP for this process is supplied from within the erythrocyte. It seemed reasonable to examine me...

1. The enzyme that catalyses the transfer of galactose from UDP-galactose to N-acetylgalactosaminyl-(1-->4)-N-acetylneuraminyl-(2-->3)-galactosyl-(1-->4)-glucosylceramide (G(M2)) was found mainly in the heavy- and light-microsomal fractions of the adult frog brain. 2. The subcellular distribution of the enzyme, UDP-galactose-G(M2) galactosyltransferase, parallels that of gangliosides in adult f...

Phosphatidylcholine synthesis by rat type II pneumonocytes was altered either by depleting the cells of choline or by exposing the cells to extracellular lung surfactant. Effects of these experimental treatments on the activity of a regulatory enzyme, CTP:phosphocholine cytidylyltransferase, were investigated. Although choline depletion of type II pneumonocytes resulted in inhibition of phospha...