Proteins A and G were each labelled with two different europium chelates (p-isothiocyanatophenyl-ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid). Their affinities for IgG from rabbit, goat, horse, sheep, mouse, pig, and rat were then measured using time-resolved fluorescence. Protein G labelled with the second chelate was found to be especially effective in binding to go...

introduction: enlargement of the root canal may potentially affect efficient smear layer (sl) removal. the aim of the present in vitro study was to compare sl removal following canal preparation with two different sizes/tapers by means of scanning electron microscopy (sem). methods and materials: a total of 50 extracted human mandibular premolars were decoronated. the teeth were randomly divide...

Two highly efficient protocols for the production of bis-coumarins under solvent-free conditions have been introduced. The reaction of 4-hydroxycoumarin (2 eq.) with arylaldehydes (1 eq.) using phthalimide-N-sulfonic acid (PhtSA) or isatin-N-sulfonic acid (IsSA), as solid-acid catalysts, afforded the mentioned compounds with good to excellent yields in short times.Briefly, we have introduced tw...

Pseudorabies virus (PRV) propagated in rabbit kidney-derived RK-13 cells (PRV-RK) was neutralized by serum obtained from specific pathogen-free pigs through the activation of complement. The virus-neutralizing activity of swine serum was lost after treatment with ethylene glycol-bis-aminoethylether-N,N,N',N'-tetraacetic acid (EGTA) or ethylenediaminetetraacetic acid (EDTA). Anti-C1q and anti-Ig...

BACKGROUND Brucella abortus can proliferate within professional and nonprofessional phagocytic host cells and thereby successfully bypass the bacteriocidal effects of phagocytes. However, the intracellular survival mechanism and factors of virulence are not fully understood. METHODS We have investigated the role of the regulator of G protein signaling 2 (RGS2), an intracellular calcium ([Ca(2...

The role of complement in host resistance to infection with Trypanosoma lewisi was studied in normal, C4-deficient, and C3-depleted rats. Complement levels were measured in normal rats throughout the course of infection. A drastic reduction of total complement and C4 hemolytic activities occurred, and C3 levels measured immunochemically were decreased. Although total complement and C4 levels we...

Previous studies from this laboratory have demonstrated that Mycobacterium leprae, an obligate intracellular bacterial parasite, enters human mononuclear phagocytes via complement receptors on these host cells and bacterium-bound C3. The present study investigates the role of M. leprae surface molecules in C3 fixation and phagocytosis. By enzyme-linked immunosorbent assay, C3 binds selectively ...

The aim of the present study was to purify and characterize a toxic protease secreted by the pathogenic Photobacterium damselae subsp. piscicida strain CP1 originally isolated from diseased cobia (Rachycentron canadum). The toxin isolated by anion exchange chromatography, was a metalloprotease, inhibited by L-cysteine, ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis(beta-aminoethyl ...

Recently, the use of superparamagnetic iron oxide nanoparticles (SPIONS) as a new and promising source of iron in agriculture has been suggested that further investigation is needed before extensive field use. In a greenhouse experiment, the effect of coated magnetite nanoparticles with humic acid (Fe3O4/HA NPs) was investigated on iron deficiency chlorosis and photosynthesis efficiency compare...