نتایج جستجو برای: explants type

تعداد نتایج: 1348137  

2015
Karina M. Basso Ana Paula F.R.L. Bracarense

Intestinal explants are a useful model for toxicological and pathological studies, but the maintenance of tissue viability for longer periods of incubation remains challenging. The aim of this study was to evaluate the effect of age on the viability of intestinal explants. Jejunal explants were obtained from 24and 42-day-old piglets. The explants were incubated for two or four hours and were th...

Journal: :Cell reports 2016
Lee Adam Wheeler Radiana T Trifonova Vladimir Vrbanac Natasha S Barteneva Xing Liu Brooke Bollman Lauren Onofrey Sachin Mulik Shahin Ranjbar Andrew D Luster Andrew M Tager Judy Lieberman

Despite their antiviral effect, the in vivo effect of interferons on HIV transmission is difficult to predict, because interferons also activate and recruit HIV-susceptible cells to sites of infection. HIV does not normally induce type I interferons in infected cells, but does if TREX1 is knocked down. Here, we investigated the effect of topical TREX1 knockdown and local interferon production o...

اصغری زکریا, رسول, زارع, ناصر, شیخ زاده مصدق, پریسا, عسکری, علی اصغر, هنرمند, لیلی,

Ultrasound has multiple industrial, medical and biotechnological applications. Ultrasound increases membrane permeability and causes several biological effects in plant cells. In this research, the effects of ultrasound on survival and growth of the sainfoin shoot apex explants were investigated. For this, shoot apex explants were exposed to ultrasonic waves (Frequency 37 kHz) for 0, 20, 30, 40...

Journal: :Development 2001
A Liu A L Joyner

Fgf8, which is expressed at the embryonic mid/hindbrain junction, is required for and sufficient to induce the formation of midbrain and cerebellar structures. To address through what genetic pathways FGF8 acts, we examined the epistatic relationships of mid/hindbrain genes that respond to FGF8, using a novel mouse brain explant culture system. We found that En2 and Gbx2 are the first genes to ...

Journal: :The Journal of endocrinology 2003
A Tumber S Papaioannou J Breckon M C Meikle J J Reynolds P A Hill

The aims of this study were to identify the role and sites of action of serine proteinases (SPs) in bone resorption, a process which involves a cascade of events, the central step of which is the removal of bone matrix by osteoclasts (OCs). This resorbing activity, however, is also determined by recruitment of new OCs to future resorption sites and removal of the osteoid layer by osteoblasts (O...

Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...

2013
Andrzej Przemysław Herman Agata Krawczyńska Joanna Bochenek Elżbieta Dobek Anna Herman Dorota Tomaszewska-Zaremba

Acting at the level of the brain, interleukin- (IL-)1 β is considered to be one of the most potent downregulators of reproduction processes during immune/inflammatory challenge. IL-1 β suppresses gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus resulting in the inhibition of the luteinizing hormone (LH) release from the anterior pituitary (AP). However, the presence of IL-1...

Journal: :The American journal of anatomy 1978
T V Fischer R H Kahn W E Burkel D W Vinter K R Herwig

Organ explants from the canine prostate with and without methylprednisolone pretreatment were cultivated for ten days in Trowell's T-8 medium or medium supplemented with testosterone and/or vitamin A. Upon termination of the experiments, explants were fixed and examined histologically. All glands from the most central section of each explant were graded according to epithelial type, and from th...

Journal: :Developmental biology 2002
Lance A Davidson Benjamin G Hoffstrom Raymond Keller Douglas W DeSimone

We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells within these explants display monopolar protrusive activity and radially interca...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2007
Valérie Haydont Céline Bourgier Marc Pocard Antoine Lusinchi Jocelyne Aigueperse Denis Mathé Jean Bourhis Marie-Catherine Vozenin-Brotons

PURPOSES Intestinal complications after radiotherapy are caused by transmural fibrosis and impair the quality of life of cancer survivors. Radiation fibrosis was considered permanent and irreversible, but recently, its dynamic nature was shown, providing new opportunities for the development of antifibrotic therapies. Among these new targets, we identified the Rho/ROCK pathway and thought to in...

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