elaboration of different toxins by enterotoxigenic e. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. various strategies have been employed to raise antibodies against these toxins as a line of defense. in this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...

background: vascular endothelial growth factor (vegf) is a coordinate regulator of physiological angiogenesis during embryogenesis, skeletal growth and reproductive functions. there are several types of vegf, including vegf165. vegfs stimulate endothelial cell growth, angiogenesis, and capillary permeability. low induction temperature is a major factor for expression of the recombinant vegf165 ...

we studied the expression of human growth hormone (hgh) in e. coli under a bacteriophage t5-base promoter in a pqe30 expression vector. for an efficient expression of hgh cdna, a number of codons at the hgh n-terminal coding region were altered based on the e. coli major codons. an over-expression of hgh in the bacteria, carrying the recombinant plasmids, was observed at 37°c in the presence of...

conclusions full factorial design experimental approach helped to identify the critical culture condition parameters in heterologous expression of v2r. it would allow studying the parameters affecting the function of this protein in future experiments. results the maximum amount of protein production was obtained by the addition of 0.75 mm iptg at 37°c after three hours. the most important fact...

Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...

Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...

protein a is a commercially important protein in biotechnological and medicinal applications. the great value of this protein and its applications in genetic and protein engineering and microbial researches as well as the growing use in biochemical industries, biotechnology, medicine and pharmacology, highlight the importance of the present study. in this survey the encoding genes of full-lengt...