Objective: Trying to diagnose clinically some suggested causes of unexplained infertility related to ovum picking, fertilization, embryo growth and implantation and to outline the most suitable management needed for these cases. Design: Prospective controlled diagnostic and management study. Setting: 50 fertile cases as control. Patients: Assessment of ovum picking was determined clinically in ...

The development of homologous functional bio-assays for sperm quality assessment has been a focal point of reproductive biologists in order to provide a scientifically- based diagnosis in cases of fertilization failure. The availability of viable oocytes still remains an important limiting factor for laboratories to embark on the methodology of assays that examine sperm-oocyte interaction. The ...

BACKGROUND Irradiation is one of the major causes of induced sperm DNA damage. Various studies suggested a relation between sperm DNA damage and fertilization rate after intra-cytoplasmic sperm injection (ICSI). OBJECTIVE In this study, fertilization rate and premature chromosome condensation (PCC) formation after ICSI of hamster oocytes with irradiated sperms from normal and oligosperm indiv...

Mature mammalian oocytes undergo a prolonged series of cytoplasmic calcium (Ca(2+)) oscillations at fertilization that are the cause of oocyte activation. The Ca(2+) oscillations in mammalian oocytes are driven via inositol 1,4,5-trisphosphate (IP3) generation. Microinjection of the sperm-derived phospholipase C-zeta (PLCζ), which generates IP3, causes the same pattern of Ca(2+) oscillations as...

The present report describes a successful intracytoplasmic sperm injection (ICSI) procedure performed with immotile spermatozoa from a young man with a combination of dysplasia of the fibrous sheath and dynein deficiency, a recently described variant of the immotile cilia syndrome. This methodology provides the only suitable solution for these patients in whom all other assisted fertilization t...

Naturally-occurring antisperm antibodies in men are a relative cause of infertility, being the fertility impairment related with the degree of sperm autoimmunization. The impairment of sperm penetration through the cervical mucus represents the best established mechanism of the antibody interference with fertility. Another mechanism may involve complement-mediated sperm injury and opsonizing ef...

interspecific or intergenus hybridization in sugarbeet (b. vulgaris l.) is usually failed due to non fertilization, embryo death or absence of connection between embryo and endosperm. consequently invitro embryo rescue technique is of value to save hybrid embryos before annihilation. sugar beet inter-specific crosses with wild species is impossible in classical breeding because of no chromosoma...

Objective: To assess the influence of MII oocyte morphology on the fertilization outcome and embryo development in ART cycles. Since, age may affect the quality of oocyte, the role of women's age was also evaluated following IVF or ICSI cycles. Materials and Methods: 200 MII oocytes from 30 IVF and ICSI cycles were evaluated. The morphology of oocytes that were fertilized in IVF cycles (IVF+) w...

Evidence-based medicine has become the golden standard of good medical practice. I analysed meta-analyses and systematic reviews, the cornerstones of evidence-based medicine, pertaining to two important problems in in vitro fertilization: failed implantation and poor ovarian response to ovarian stimulation. Numerous interventions and procedures have been tried to facilitate implantation and to ...