Dye-diffusion test for semiquantitative staphylocoagulase assay. Ever since 1903, when Leob reported the ability of staphylococci to produce coagulase, an enzyme capable of clotting plasma, numerous workers have demonstrated the practical applicability of this phenomenon as an indicator of potential pathogenicity of these organisms. Two general procedures for coagulase determination are the tub...

The use of resins as a basis for "tubeless test meals " has made the unpleasant experience of aspiration of gastric contents unnecessary in many cases. A substance incorporated in the resin is released in the stomach only in the presence of free hydrochloric acid. This substance is then absorbed into the blood and excreted in the urine. Originally quinine was used in the test, and estimated flu...

There are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as confirmatory tests for samples identified as positive or equivocal by an electrochemiluminescent immunoassay (ECLIA) were examined. Cut-off values were...