Iron isotopes have been extensively used to trace the history of microbial metabolisms and the redox evolution of the oceans. Archean sedimentary rocks display greater variability in iron isotope ratios and more markedly negative values than those deposited in the Proterozoic and Phanerozoic. This increased variability has been linked to changes in either water column iron cycling or the extent...

It is unknown to which extent phototrophic Fe(II) oxidation takes place in the simultaneous presence of organic electron donors (e.g., acetate/lactate). Therefore, the photoferrotrophic strain Rhodopseudomonas palustris TIE-1 was inoculated with various combinations of Fe(II), acetate and lactate to understand metabolic substrate preference. When acetate was provided together with Fe(II), TIE-1...

I This contribution is one of a series of papers by the Multiple Fellowship on Mine Acid Control sustained at Mellon Institute by the Sanitary Water Board, Department of Health, Commonwealth of Pennsylvania. catalyst. Indeed, Carpeniter and Heirndon (1933) suggested that bacterial activity by some sulfur oxidizing organism might explain, in part, the high acidities usually encountered in bitumi...

I This contribution is one of a series of papers by the Multiple Fellowship on Mine Acid Control sustained at Mellon Institute by the Sanitary Water Board, Department of Health, Commonwealth of Pennsylvania. catalyst. Indeed, Carpeniter and Heirndon (1933) suggested that bacterial activity by some sulfur oxidizing organism might explain, in part, the high acidities usually encountered in bitumi...

Oxidation of ferrous iron by Thiobacillus ferrooxidans SM-4 was inhibited competitively by increasing concentrations of ferric iron or cells. A kinetic analysis showed that binding of one inhibitor did not exclude binding of the other and led to synergistic inhibition by the two inhibitors. Binding of one inhibitor, however, was affected by the other inhibitor, and the apparent inhibition const...

Photoferrotrophy is presumed to be an ancient type of photosynthetic metabolism in which bacteria use the reducing power of ferrous iron to drive carbon fixation. In this work the putative iron oxidoreductase of the photoferrotroph Rhodobacter ferrooxidans SW2 was cloned, purified, and characterized for the first time. This protein, FoxE, was characterized using spectroscopic, thermodynamic, an...

Iron in the early anoxic oceans of Archean age (4000-2500 million years ago) is believed to have been oxidized form banded iron formations (BIF). Previously, it has proposed that was either by free oxygen, H2O2, microbial oxidation, or photo-oxidation. However, these mechanisms are difficult reconcile with evidence for at time having largely devoid dissolved oxygen and oxidants, together rarity...

The type strain (ATCC 23270) and two other strains of Thiobacillus ferrooxidans were able to grow by hydrogen oxidation, a feature not recognized before. When cultivated on H2, a hydrogenase was induced and the strains were less extremely acidophilic than during growth on sulfidic ores. Cells of T. ferrooxidans grown on H2 and on ferrous iron showed 100% DNA homology. Hydrogen oxidation was not...