The presence of various cytokines, namely the tumor necrosis factor (TNF-alpha), interferon (IFN-gamma), and interleukins (IL-1 beta and IL-6), was investigated in seminal plasma of fertile, infertile, and immunoinfertile men using specific immunoradiometric assays. TNF-alpha and IL-1 beta were not detected. IFN-gamma was detected, but the differences between the levels of fertile and infertile...

Testicular biopsy specimens from 16 fertile and 10 subfertile men with normal male karyotype were studied quantitatively to provide histological and cytogenetic data for a basis of reference in assessing abnormalities of spermatogenesis. Histological studies included estimation of the proportion and activity of germinal epithelium and an assessment of tubular morphology. In cytogenetic preparat...

BACKGROUND The sperm chromatin of fertile men retains a small number of nucleosomes that are enriched at developmental gene promoters and imprinted gene loci. This unique chromatin packaging at certain gene promoters provides these genomic loci the ability to convey instructive epigenetic information to the zygote, potentially expanding the role and significance of the sperm epigenome in embryo...

: Presence of antisperm antibodies (ASAs) in infertility and their adverse effects on fertility is a matter of controversy. The aims of this study were to determine the percentage of antibody positive sperms and rate of ASA positive sera in both fertile and infertile men and women, and to find the association between these antibodies and infertility. Methods: This study consisted of 29 fertile ...

Using an enzyme-linked immunosorbent assay (ELISA), sera (n = 15) and seminal plasma (n = 30) from antisperm antibody-positive immunoinfertile men (n = 45) and from fertile men (n = 45), were tested for the immunoreactivity with the synthetic YLP(12) sperm peptide. Of the 15 immunoinfertile sera tested, 46% were positive for immunoglobulin (Ig)M, 73% for IgG, and 40% for IgA. Of the 30 samples ...

The aim of this study was to assess the level of malondialdehyde (MDA) in the seminal plasma of infertile men and to highlight a relationship between the level of MDA and semen parameters. Eighty-one infertile patients were divided into groups according to their clinical diagnosis: genitourinary infections, varicocele and idiopathic infertility. Semen quality was assessed by light and transmiss...

background: reactive oxygen species (ros)-induced lipidperoxidation can lead to dysfunction of sperm and thereby, infertility may be occurred. so, always there is a balance between amount of ros and anti-oxidant molecules in semen. anti-oxidant enzymes of sperm; superoxide dismutase (sod), glutathione peroxidase (gpx), catalse and zinc and selenium can protect it from destructive effects of ros...

Background: Defective sperm function is now recognized as one of the most important causes of male infertility. Lipid peroxidation (LPO) is known to cause various impairments of sperm cells and may play a major role in the etiology of male infertility. Malondialdehyde (MDA) is one of the final products of lipid peroxidation in seminal plasma. Seminal plasma possesses a rich source of different ...

Background: Phospholipids are distributed asymmetrically between inner and outer leaflets of the plasma membrane of live cells. Early during apoptosis, this asymmetry is disrupted and phosphatidylserine becomes exposed on the outside surface of the plasma membrane. There is little information about the effects of vitrification on apoptosis. Objective: The aim of the present study was to evaluat...