The in situ distribution of the 26-kdalton Main Intrinsic Polypeptide (MIP or MP 26), a putative gap junction protein in ocular lens fibers, was defined at the electron microscope level using indirect immunoferritin labeling of ultrathin frozen sections of rat lens. MIP was found distributed throughout the plasma membrane of the lens fiber cell, with no apparent distinction between junctional a...

Solvent extraction of copper in hollow-fiber membrane contactors was investigated in this study. Extraction of copper is carried out conventionally using mixer-settlers. These contactors have some disadvantages such as consumption of high energy, dispersion of two phases and high operating costs. Membrane contactors can obviate the need for conventional contactors. The most favorable module use...

Hexagonal packing geometry is a hallmark of close-packed epithelial cells in metazoans. Here, we used fiber cells of the vertebrate eye lens as a model system to determine how the membrane skeleton controls hexagonal packing of post-mitotic cells. The membrane skeleton consists of spectrin tetramers linked to actin filaments (F-actin), which are capped by tropomodulin1 (Tmod1) and stabilized by...

PURPOSE To test the ability of lens fiber cells at various stages of differentiation to transcribe and translate microinjected DNA templates. METHODS Expression plasmids encoding green fluorescent protein (GFP) or a GFP-tagged membrane protein (human CD46) were microinjected into organ-cultured embryonic chicken lenses. Protein expression was visualized by confocal microscopy. RESULTS GFP e...

Membrane electrical properties were measured in sheep cardiac Purkinje fibers, having diameters ranging from 50 to 300 mum. Both membrane capacitance and conductance per unit area of apparent fiber surface varied fourfold over this range. Membrane time constant, and capacitance per unit apparent surface area calculated from the foot of the action potential were independent of fiber diameter, ha...

nowadays, membrane processes are widely used for high purity nitrogen production. separation of air by membranes is a flexible process in which the investment and operational costs are lower than for example adsorption or cryogenic processes. such processes are based on relative permeability of air components which are transported by the pressure difference between two sides of the membrane. ma...

PURPOSE The immunoglobulin superfamily member Cadm1 is a single-pass, type 1 membrane protein that mediates calcium-independent, cell-cell adhesion. Cadm1 has been implicated in tumor formation and synaptogenesis. A recent analysis of mouse lens cell membranes identified Cadm1 as a major constituent of the fiber cell membrane proteome. Here the authors examined the expression and function of Ca...

A novel cation exchange fiber supported solid membrane was developed for transport of cobalt, manganese and nickel metal ions. Fiber supported solid membrane was prepared by chemically modifying the cellulose fibers with citric acid. In the fiber supported solid membrane, the diffusion by capillary action by keeping the liquid phase in the porous solid fiber and ion exchange mechanism were used...

Nowadays, membrane processes are widely used for high purity Nitrogen production. Separation of air by membranes is a flexible process in which the investment and operational costs are lower than for example adsorption or cryogenic processes. Such processes are based on relative permeability of air components which are transported by the pressure difference between two sides of the membrane. M...