BACKGROUND Generation of an effective dendritic cell (DC) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. OBJECTIVE To compare the effects of monocyte separation methods, flask adherence (Flask-DC) and magnetic activated cell sorting (MACS-DC), on phenotypic and functional characteristics of resultant DCs. METHODS DCs from healthy volunteers were generated...

A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic inc...

An improved flask system for the growth of extremely oxygen-sensitive bacteria in liquid culture is described. The improvement described utilizes an all-glass, neoprene-stoppered flask designed for growth of 50- to 1,000-ml cultures of bacteria with continuous gassing.

Chinese hamster ovary (CHO) cells is one of the most widely used production host for the commercial production of biopharmaceuticals product. They have been extensively studied and developed, and today provide a stable platform for producing monoclonal antibodies and recombinant proteins. This study focused on the production of recombinant protein in suspension culture of CHO cells in spinner f...

BACKGROUND Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate. Determination and optimization of these parameters through experiments are labor-intensive and time-consuming. Computational Fluid Dynamics (CFD) provides the ability to p...

PT XYZ adalah perusahaan di Indonesia yang bergerak dalam bidang retail memiliki lebih dari 14.300 toko serta 32 cabang atau warehouse. Masalah muncul ketika telah menentukan harga suatu barang dan harus disebarkan ke setiap toko. Harga akan selalu berubah karena adanya promo diskon masa periode nya sendiri, sehingga diperlukan penyebaran secara real-time. Selain itu, dengan jutaan transaksi ti...