Cytokine flow cytometry (CFC) is a general term that applies to flow cytometric analysis of cells using anticytokine antibodies as markers of activation. The most common version of this technique is the intracellular staining of cytokines in cells that have been fixed and permeabilized after short-term in vitro activation. When used with specific antigens, this technique allows for the quantita...

Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing, and analyzing flow cytometry data using extensive computational resources and tools. Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. Flow cytometry a...

Cellular measurements by flow cytometric analysis constitute an important step toward understanding individual attributes within a population of cells. Assessing individual cells within a population by protein expression using fluorescently labeled antibodies and other fluorescent probes can identify cellular patterns. The technology for accurately identifying subtle changes in protein expressi...

The title of this book, Practical Flow Cytometry, may be somewhat misleading; a significant portion of the information presented is not essential to most investigators who utilize flow cytometry. The author's broad expertise in electronics, optics, histochemistry, and biology provides an excellent basis for discussion of the principles of flow cytometry and cell sorting; however, successful app...

Figure 1: Schematic of the particles in the fluid flow and interaction region of the laser within the flow cell. A key and continuing goal in the development of flow cytometry techniques is the ability to measure ever more parameters for each particle under test. Work carried out by Prof John Nolan’s group at La Jolla Bioengineering Institute, and reported recently by Watson et. al.[1] in Cytom...

Background: Invariant natural killer cells (iNKT) are an important immunoregulatory T cell subset. Currently several flow cytometry-based approaches exist for the identifi-cation of iNKT cells, which rely on using the 6B11 monoclonal antibody or a combina-tion of anti-Vα24 and anti-Vβ11 antibodies. Objective: The aim of this study was to compare the ability of two flow cytometry-based methods f...