Energy transfer (ET) from excited matrix to fluorescent traps is used to probe the mobility of excitations in the matrix-assisted laser desorption/ionization (MALDI) matrix material 2,5-dihydroxybenzoic acid. The dependence of host and guest fluorescence on excitation density (laser intensity) and trap concentration gives clear evidence for long-range energy transport in this matrix. This concl...

.a simple spectrofluorimetric method is described for the determination of doxorubicin (dxr) based on its quenching effect on the fluorescence intensity of tb 3+ - deferasirox (dfx) complex as a fluorescent probe. the excitation and emission wavelengths were 328 and 545 nm, respectively. the effects of ph, time, order of addition of reagents, concentrations of tb 3+ and dfx and the buffer volum...

The purpose of this short communication is to demonstrate the importance of numerical analysis and wavelength increment selection when characterizing fluorescent dissolved organic matter (FDOM) using fluorescence excitation-emission matrix (EEM) regional integration. A variety of water samples, representing a landscape gradient and different types of FDOM, were analyzed for their percentage dis...

We demonstrate how the anisotropy of the vacuum of the electromagnetic field can lead to quantum interferences among the decay channels of close lying states. Our key result is that interferences are given by the scalar formed from the antinormally ordered electric field correlation tensor for the anisotropic vacuum and the dipole matrix elements for the two transitions. We present results for ...

Poster 1 A REAL-TIME EXCITATION-EMISSION MATRIX FLUORESCENCE SPECTROPHOTOMETER FOR IN VIVO SKIN TISSUE DIAGNOSIS Wenbo Wang, Jianhua Zhao, Harvey Lui, and Haishan Zeng Photomedicine Institute – Department of Dermatology and Skin Science, Vancouver Coastal Health Research Institute and University of British Columbia, 835 West 10th Avenue, Vancouver, BC, Canada V5Z 4E8; Imaging Unit – Integrative...

Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the contrast of a fluorescence image using an OIM slide, a linearly-polarized excitation light was employed, and...

We isolated and characterized a green fluorescent protein (GFP) from the sea cactus Cavernularia obesa. This GFP exists as a dimer and has absorption maxima at 388 and 498 nm. Excitation at 388 nm leads to blue fluorescence (456 nm maximum) at pH 5 and below, and green fluorescence (507 nm maximum) at pH 7 and above, and the GFP is remarkably stable at pH 4. Excitation at 498 nm leads to green ...

Dextromethorphan (DEX) is an antitussive agent used in many cough and cold medications, and dextrorphan (DOR) is its metabolite. Owing to their similar structures, optimization of the condition for the chromatography approach, which is in common use for determination, is both demanding and time-consuming. This paper describes a methodology that combines excitation-emission matrix fluorescence s...

We present a computationally efficient and accurate adjoint method for calculating coupled sensitivities of complex frequency-domain excitation and emission fluence to any underlying optical parameters in highly scattering media. The method is shown to be general and accurate. Novel vectorized implementations for finite element global matrix assembly and adjoint sensitivity calculations are sho...

Many naturally occurring substances are intrinsically fluorescent (autofluorescent) when excited with UV or visible wavelengths. Autofluorescence emission typically spans the visible spectrum with a lifetime (?) measured in nanoseconds. Time-resolved fluorescence techniques exploit the large difference in ? that exists between typical autofluorophores and for example, lanthanide chelates (typic...