Fluorescent and colorimetric reporter genes are valuable tools for drug screening models, since microscopy is labor intensive and subject to observer variation. In this work, we propose a fluorimetric method for drug screening using red fluorescent parasites. Fluorescent Leishmania amazonensis were developed after transfection with integration plasmids containing either red (RFP) or green fluor...

A high-performance liquid chromatographic (HPLC) method is described for the determination of duloxetine hydrochloride in capsules. The method was based on pre-column derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole using the fluorimetric detection technique. Duloxetine hydrochloride was analyzed by HPLC using an Inertsil C18 column (5 μm, 150 × 4.6 mm) and mobile phase consisted of ...

Background: Uranium poses both chemical and radiological hazard to the living system. Drinking water from river is one of the major sources of uranium intake. Dissolution of minerals, washout from rain water, rock-water interaction, agricultural run off, and industrial disposals are some of the sources of uranium in river water system. Present study was aimed to determine the uranium in the wat...

The determination of radium and/or uranium concentrations in natural waters is useful in a wide range of environmental studies. In this work, a low-background liquid scintillation system (Wallac, Quantulus 1220) was used to determine simultaneously 226Ra and uranium (total) activities in natural water samples. Sample preparation involved filtration and 1 : 10 concentration by evaporation. Then,...

A portable instrument for oxygen determination, based on the quenching of phosphorescent octaethylporphyrin by gaseous O(2), has been developed using the fluorimetric paired emitter-detector diode technique (FPEDD). The instrument configuration consists of two light-emitting diodes (LEDs) facing each other, with an interchangeable support containing a phosphorescent membrane in between, in whic...

We describe a method for the measurement of protein-bound malondialdehyde with the thiobarbituric acid reaction in human plasma using second-derivative spectrophotometry. Calibration was done by spectrum height measurement from the baseline at 532 nm. The data were compared with those obtained by using conventional absorbance and fluorimetric measurements. The results were linear from 0.2 to 80...

MicroRNAs (miRNAs) are small endogenous non-coding RNAs of ~22 nucleotides that play important functions in the regulation of many biological processes, including cell proliferation, differentiation, and death. Since their expression has been in close association with the development of many diseases, recently, miRNAs have been regarded as clinically important biomarkers and drug discovery targ...

At submicromolar concentrations two novel phenanthridine biguanides exhibit distinctly different spectroscopic signals for dGdC and dAdT sequences, respectively, by opposite fluorimetric changes (quenching for dGdC and increase for dAdT) and especially the bis-biguanide derivative gives an opposite ICD response (negative ICD for dGC and strong positive for dAdT). This specific signalling was ex...