Calcium flux is a rapid and sensitive measure of cell activation whose utility could be enhanced with better techniques for data extraction. We describe a technique to monitor calcium flux by flow cytometry, measuring Fura Red calcium dye by ratiometric analysis. This technique has several advantages: 1) using a single calcium dye provides an additional channel for surface marker characterizati...

Conessine, a steroidal alkaloid isolated from Holarrhena floribunda, has anti-malarial activity and interacts with the histamine H3 receptor. However, the cellular effects of conessine are poorly understood. Accordingly, we evaluated the involvement of conessine in the regulation of autophagy. We searched natural compounds that modulate autophagy, and conessine was identified as an inhibitor of...

this paper presents a sensorless system drive on primary flux oriented control (pfoc) and secondary flux oriented control (sfoc) for the linear induction motor (lim) with taking into account end effect. extended kalman filter (ekf) is applied to estimate lim speed by measuring motor voltages and currents. in order to achieve desirable dynamic and robustness motor performance instead of traditio...

Based on a combination of clinical and pedagogical experience in occupational therapy, which interlinks artistic practices and therapeutic processes, we carried out a historical study designed to investigate how the fields of mental health and art became interrelated in Brazil between the mid 19th and 20th centuries. The methods used for this research, which is underpinned by the thinking of Fo...

single-phase squirrel cage and three-phase induction electromotors are the most popular electromotors used in industry from long ago. when an induction motor starts with direct connection method, it takes a lot of current from the power supply (several times the nominal current); so, several methods have been devised to start such electromotors. these methods either reduce the voltage of the st...

The goal of this project was to measure directly the volatilization of gas phase polychlorinated biphenyls (PCBs) from stabilized dredged material (SDM) in a • laboratory scale flux chamber. This study complements earlier field observations (Phase I) to estimate PCB fluxes from SDM being applied to a landfill (Korfiatis et. al., 2003, Miskewitz et al, 2004). Phase I included measurements of the...

Cogging torque is the major limitation of axial flux permanent magnet motors. The reduction of cogging torque during the design process is highly desirable to enhance the overall performance of axial flux permanent magnet motors. This paper presents a double-layer magnet design technique for cogging torque reduction of axial flux permanent magnet motor. Initially, 250 W, 150 rpm axial flux brus...

In order to investigate the influence of aflatoxin B1 (AFB1) on intestinal bacterial flora, 24 Kunming mice (KM mice) were randomly placed into four groups, which were labeled as control, low-dose, medium-dose, and high-dose groups. They were fed intragastrically with 0.4 mL of 0 mg/L, 2.5 mg/L, 4 mg/L, or 10 mg/L of AFB1 solutions, twice a day for 2 months. The hypervariable region V3 + V4 on ...