Two strategies are described for detecting constitutive or induced protein-protein interactions in intact mammalian cells; these strategies are based on oligomerization domain-assisted complementation of rationally designed fragments of the murine enzyme dihydrofolate reductase (DHFR; EC 1.5.1.3). We describe a dominant clonal-selection assay of stably transfected cells expressing partner prote...

This study aimed to determine total antioxidant and antioxidant activity of selected local varieties of lettuce (Lactuca sativa L.). Five varieties (iceberg, butterhead, romaine, green coral and red coral) were subjected to DPPH radical scavenging activity and ferric reducing antioxidant power assay (FRAP) assays for determination of antioxidant activity. Total phenolic content and total flavon...

1. Rice-Evans C, Miller NJ. Total antioxidant status in plasma and body fluids. Methods Enzymol 1994;234:279–93. 2. Cao G, Alessio CP, Cutler RG. Oxygen-radical absorbance capacity assay for antioxidants. Free Radic Biol Med 1993;14:303–11. 3. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996;239: 70–6. 4. ...

Piper chaba Hunter, a flowering vine of Piperaceae family, has been using for long time in south Asian country for culinary meat preparation. As stem and root of this plant has been using traditionally in fat rich meat preparation, the antioxidant and anticoagulation potential of this herb is a great interest of study and it was evaluated using cold and boiled water extract of dried and grinded...

It would be desirable to establish and standardize methods that can measure the total antioxidant capacity level directly from vegetable extracts containing phenolics. Antioxidant capacity assays may be broadly classified as electron transfer (ET)- and hydrogen atom transfer (HAT)-based assays. The majority of HAT assays are kinetics-based, and involve a competitive reaction scheme in which ant...

Madhuca longifolia (Maddhu) and Ficus racemose (Attikka) are well known medicinal plants which have been valued for decades in Sri Lankan Ayurvedic medicine. This research was aimed to study the phytochemical analysis evaluate invitro antioxidant activity of two solvent extracts (70% aqueous acetone 80% methanol) obtained from each bark selected plants. The crude were prepared by steeping dried...

New sulfonamide and amide derivatives containing coumarin moieties; oxo-2H-chromen-sulfamoylphenylacetamides and oxo-2H-chromen-arylacetamides were synthesized starting from diverse 2-chloroacetamide derivatives and a wide range of coumarins. The structures of the obtained compounds were elucidated by IR and NMR spectra and also analytical elemental analysis. In the next step, the above mention...

New sulfonamide and amide derivatives containing coumarin moieties; oxo-2H-chromen-sulfamoylphenylacetamides and oxo-2H-chromen-arylacetamides were synthesized starting from diverse 2-chloroacetamide derivatives and a wide range of coumarins. The structures of the obtained compounds were elucidated by IR and NMR spectra and also analytical elemental analysis. In the next step, the above mention...

Cadmium (Cd) contamination is a highly dangerous international problem because it can transfer into the food chain and become bioaccumulated, endangering human health. Cd detoxication is very interesting particularly the method providing no undesirable side effects. Cd also causes lipid oxidation that leads to undesired food quality. Garlic (Allium sativum L.) has been used as conventional food...

Herbal water is referred to the liquid obtained from the distillation of medicinal plants. Different parts of plants, such as flowers, fruits, leaves, seeds and roots have long been used to produce herbal waters. Herbal waters are used as dietary supplements and alternative medicine and are commonly used for flavoring in baking. Previous studies focused on the non-volatile constituents of herbs...