1. The antigenicity of four soya-bean-based infant formulas (Prosobee powder, Prosobee liquid concentrate (Mead Johnson, Uxbridge, Middx), Wysoy (Wyeth, Maidenhead, Berks) and Formula S (Cow and Gate, Trowbridge, Wilts] was measured by enzyme-linked immunosorbent assays (ELISAs) specific for glycinin and beta-conglycinin. Results were compared with in vivo assessments of antigenicity using guin...

objective the goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. materials and methods microspheres were prepared by an emulsification technique and characterized for size, encapsulation efficiency, and release profile of encapsulates. selection of appropriate parameters (viscosity of algina...

conclusions results showed that hcvcp + bcg induced a moderate ctl and mixed th1/th2 immune responses with higher levels of cell proliferation and ifn-γ secretion, indicating that bcg may have a better outcome when formulated in hcvcp-based subunit vaccines. results expression and purification of core protein around the expected size (21 kda) was confirmed by western blotting. the hcvcp + bcg v...

methods mycobacterium tuberculosis standard strain crbip7.11 was cultured at 37°c in loewenstein johnson (lj) media for 3-4 weeks. to confirm the species, standard microbiological and biochemical tests were performed. after preparation of membrane vesicles, the amount of protein in membrane vesicles was measured by sds-page and nanodrop. to analyze the integrity and morphology of extracellular ...

an oil adjuvant vaccine (oav) was prepared from a local strain of pasteurella multocida. strain 6:b was cultured and inactivated by formalin. bacterial pellet was prepared by centrifugation and subsequently adjuvanted by montannide oil isa-70. a dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by im route. animals were bled before and at 24, 90, 150, and...

introduction: long term complications of hepatitis c virus (hcv) infection include fibrosis, cirrhosis, and hepatocellular carcinoma and although the disease is treatable with the newly introduced direct acting antivirals, but factors such as drug resistance, viral genotype and adverse effects can limit the effectiveness of therapy. therefore, development of effective, safe and affordable proph...

Abstract Background and Aims: Vaccination of poultry has a major impact on the prevention and control of avian influenza viruses. Nanobiotechnology techniques provide a new approach for improvement of influenza vaccine efficacy. In this study, efficacy of an inactivated nano-adjuvant vaccine developed based on an endemic H9N2 virus was evaluated in SPF chickens. Materials and Methods: I...

Background: Adjuvants are used to increase the immunogenicity of new generation vaccines, especially those based on recombinant proteins. Despite immunostimulatory properties, the use of bacterial lipopolysaccharide (LPS) as an adjuvant has been hampered due to its toxicity and pyrogenicity. Brucella abortus LPS is less toxic and has no pyrogenic properties compared to LPS from other gram nega...

purpose : to evaluate the effectiveness of chemoreduction (crd) for globe saving in patients with intraocular retinoblastoma methods : in this interventional case series all patients with intraocular retinoblastoma were included and classified according to the international classification of retinoblastoma (icrb) from group a to e. six cycles of intravenous vincristine, etoposide and carboplati...

introduction: the activity of hypothalamus-pituitary-adrenal (hpa) axis is increased following inflammation due to its closed relation with immune system. this axis indicates an increased secretion of acth, and corticosterone during acute inflammation while little is known about its activity during chronic inflammation such as rheumatoid arthritis (ra). in this study we measured the products of...